Transcriptomics

Dataset Information

67

MicroRNA profiling of human hepatocellular carcinoma samples shows miR-221 overexpression contributes to liver tumorigenesis


ABSTRACT: We profiled miRNA expression in tissue samples (104 HCC, 90 adjacent cirrhotic livers, 21 normal livers) as well as in 35 HCC cell lines. A set of 12 miRNAs (including miR-21, miR-221/222, miR-34a, miR-519a, miR-93,miR-96, and let-7c) was linked to disease progression from normal liver through cirrhosis to full-blown HCC. miR-221/222, the most upregulated miRNAs in tumor samples, are shown to target the CDK inhibitor p27 and to enhance cell growth in vitro. Conversely, these activities can be efficiently inhibited by an antagomiR specific for miR-221. In addition, we show, using a mouse model of liver cancer, that miR-221 overexpression stimulates growth of tumorigenic murine hepatic progenitor cells.

ORGANISM(S): Homo sapiens  

TISSUE(S): Liver, Tumor, Unspecified

DISEASE(S): Cirrhosis,Normal,Hepatocellular Carcinoma

SUBMITTER: Stefano Volinia  

PROVIDER: E-TABM-866 | ArrayExpress | 2009-12-08

REPOSITORIES: ArrayExpress

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miR-221 overexpression contributes to liver tumorigenesis.

Pineau Pascal P   Volinia Stefano S   McJunkin Katherine K   Marchio Agnès A   Battiston Carlo C   Terris Benoît B   Mazzaferro Vincenzo V   Lowe Scott W SW   Croce Carlo M CM   Dejean Anne A  

Proceedings of the National Academy of Sciences of the United States of America 20091215 1


MicroRNA (miRNAs) are negative regulators of gene expression and can function as tumor suppressors or oncogenes. Expression patterns of miRNAs and their role in the pathogenesis of hepatocellular carcinoma (HCC) are still poorly understood. We profiled miRNA expression in tissue samples (104 HCC, 90 adjacent cirrhotic livers, 21 normal livers) as well as in 35 HCC cell lines. A set of 12 miRNAs (including miR-21, miR-221/222, miR-34a, miR-519a, miR-93, miR-96, and let-7c) was linked to disease p  ...[more]

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