Unknown

Dataset Information

0

Comparison of transcriptional response of induced pluripotent stem iPS cell derived and monocyte derived macrophages to bacterial lipopolysaccharide stimulation


ABSTRACT: In the first part of this project, we will differentiate IPS cells from 5 human donors into macrophages, and extract RNA from unstimulated and LPS stimulated macrophages to perform RNA sequencing. We will also extract RNA before and after stimulation in blood- derived macrophages from 5 additional, unrelated healthy samples. In the second part of the project, RNA-seq data will be analysed to compare LPS response of these two macrophage populations. In summary, we will perform 75bp PE RNA-seq on 20 samples (10 pre and post stimulus), on the HiSeq 2500 platform. Samples will be multiplexed at 5 samples / lane, so we will require 4 flow cells in total.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

ORGANISM(S): Homo sapiens

PROVIDER: E-ERAD-192 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

2016-05-17 | E-ERAD-192 | ArrayExpress
| EGAS00001000563 | EGA
| EGAD00001001106 | EGA
| E-ERAD-132 | BioStudies
| E-ERAD-236 | BioStudies
| E-ERAD-84 | BioStudies
| E-ERAD-363 | BioStudies
| E-GEOD-32574 | BioStudies
| E-ERAD-278 | BioStudies
| E-ERAD-305 | BioStudies