Dataset Information


CRL4(CRBN) ubiquitin ligase profiling

ABSTRACT: In the 1950s the drug thalidomide administered as a sedative to pregnant women led ot the birth of thousands of children with multiple defects. Despite its teratogenicity, thalidomide and ist IMiD derivatives recently emerged as effective treatments for multiple myeloma and 5q-dysplasia. IMiDs target the CUL4-RBX1-DDB1-CRBN (CRL4(CRBN)) ubiquitin ligase. Through an unbiased screen we identify the homeobox trranscription factor MEIS2 as an endogenous substrate of CRL4(CRBN). By definition, a specific target of CRL4(CRBN) is expected to have a very low intensity on negative control arrays (E1_E2), (E1_CRBN), (E1_E2_Cdt2), (E1_E2_Cdt2_revlimid), (E1_E2_Cdt2_CSN) or with CRL4(CRBN) in presence of inhibitor (E1_E2_CRBN_revlimid) and high intensity on arrays with CRL4(CRBN) (E1_E2_CRBN) or CRL4(CRBN) in presence of CSN (E1_E2_CRBN_CSN) Accordingly 16 protein microarrays were subjected to in vitro ubiquitylation using the following enzyme combinations: 3x Uba1+UbcH5a; 2x Uba1+UbcH5a+CRL4(DDB2); 3x Uba1+UbcH5a+CRL4(CRBN); 2x Uba1+UbcH5a+CRL4(CRBN)+lenalidomide; 2x Uba1+UbcH5a+CRL4(CRBN)+CSN; 2x Uba1+UbcH5a+CRL4(Cdt2); 1x Uba1+UbcH5a+CRL4(Cdt2)+CSN; 1x Uba1+UbcH5a+CRL4(Cdt2)+lenalidomide

SUBMITTER: Eric Fischer 

PROVIDER: E-GEOD-57554 | BioStudies | 2014-05-13


REPOSITORIES: biostudies

Similar Datasets

2014-05-13 | E-GEOD-57554 | ArrayExpress
2014-01-01 | S-EPMC4423819 | BioStudies
2016-01-01 | S-EPMC4889030 | BioStudies
2020-01-01 | S-EPMC6972723 | BioStudies
2019-01-01 | S-EPMC6501120 | BioStudies
2015-01-01 | S-EPMC4853910 | BioStudies
2019-01-01 | S-EPMC7179951 | BioStudies
1000-01-01 | S-EPMC1188016 | BioStudies
2009-01-01 | S-EPMC2744448 | BioStudies
2012-01-01 | S-EPMC3322809 | BioStudies