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Purification and subunit structure of phosphoglycerate dehydrogenase from rabbit liver.


ABSTRACT: D-3-Phosphoglycerate dehydrogenase (EC 1.1.1.95) was purified from rabbit liver by (NH4)2SO4 fractionation, DEAE-Sephacel chromatography, affinity chromatography on AMP-agarose and molecular-sieve h.p.l.c. The purified enzyme was homogeneous as judged by SDS/polyacrylamide-slab-gel electrophoresis. On the basis of molecular-sieve h.p.l.c. and SDS/polyacrylamide-gel electrophoresis, the enzyme is a tetramer composed of subunits of Mr 60,000.

SUBMITTER: Lund K 

PROVIDER: S-EPMC1147222 | BioStudies | 1986-01-01

REPOSITORIES: biostudies

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