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Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii.


ABSTRACT: Glyoxylate dehydrogenase (glyoxylate:NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per active site.

SUBMITTER: Balmforth AJ 

PROVIDER: S-EPMC1153313 | BioStudies | 1984-01-01

SECONDARY ACCESSION(S): 10.1042/bj2180113

REPOSITORIES: biostudies

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