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The inactivation of rat adipocyte Mg2+-dependent phosphatidate phosphohydrolase by noradrenaline.


ABSTRACT: The inactivation of rat adipocyte Mg2+-dependent phosphatidate phosphohydrolase by noradrenaline [Cheng & Saggerson (1978) FEBS Lett. 87, 65--68; Cheng & Saggerson (1978) FEBS Lett. 93, 120--124] persists for at least 40 min in crude defatted homogenates kept at 0 degrees C or 20 degrees C, but is diminished at 37 degrees C. The effect of noradrenaline persists through the isolation of post-105000 g supernatants and is then stable in these preparations at 0 degrees C and 37 degrees C. Inclusion of albumin (10--20 mg/ml) in homogenization buffers abolishes the effect of noradrenaline. The effect of noradrenaline is not removed by dialysis of extracts or by raising the concentrations of Mg2+ or phosphatidate in assays.

SUBMITTER: Cheng CH 

PROVIDER: S-EPMC1162144 | BioStudies | 1980-01-01

SECONDARY ACCESSION(S): 10.1042/bj1900659

REPOSITORIES: biostudies

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