Dataset Information


The catabolism of intravenously injected heparan N-[35S] sulphate in the rat.

ABSTRACT: The metabolic fate of heparan N-[(35)S]sulphate was studied in rats. Heparan sulphate was obtained from either bovine aorta or lung and labelled with (35)S by desulphation and subsequent resulphation in vitro. Experiments in which heparan N-[(35)S]sulphate was administered intravenously to either free-range or wholly anaesthetized rats with ureter cannulae established that substantial desulphation occurs in vivo, with elimination of inorganic [(35)S]sulphate in urine. Oligosaccharides labelled with (35)S, possible intermediates in heparan sulphate degradation, could not be detected in urine or blood. The general distribution of radioactivity after administration of heparan N-[(35)S]sulphate, as demonstrated by whole-body radioautography, suggested that desulphation was not restricted to one organ in particular. Support for this view was obtained in experiments in which heparan N-[(35)S]sulphate was administered to animals after the removal of kidneys, liver, spleen, pancreas or gastrointestinal tract. In all cases inorganic [(35)S]sulphate was still produced. The ability of rats of desulphate heparan N-[(35)S]sulphate was progressively impaired by increasing concentrations of heparin administered simultaneously. It was concluded that heparan sulphate is metabolized at a number of sites in the body by a sequence of degradative events leading to the formation of inorganic sulphate. It is also concluded that at least some of these events are common to heparan sulphate and heparin.


PROVIDER: S-EPMC1165019 | BioStudies | 1977-01-01

SECONDARY ACCESSION(S): 10.1042/bj1660373

REPOSITORIES: biostudies

Similar Datasets

1999-01-01 | S-EPMC1220705 | BioStudies
1971-01-01 | S-EPMC1176931 | BioStudies
1973-01-01 | S-EPMC1165803 | BioStudies
1972-01-01 | S-EPMC1174511 | BioStudies
1977-01-01 | S-EPMC1164759 | BioStudies
1995-01-01 | S-EPMC1136251 | BioStudies
1985-01-01 | S-EPMC1152670 | BioStudies
1985-01-01 | S-EPMC1144886 | BioStudies
1965-01-01 | S-EPMC1206793 | BioStudies
1994-01-01 | S-EPMC1137367 | BioStudies