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Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment.


ABSTRACT: A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.

SUBMITTER: Gray M 

PROVIDER: S-EPMC194946 | BioStudies | 2003-01-01

REPOSITORIES: biostudies

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