Dataset Information


The cellular proto-oncogene product Myb acts as transcriptional activator of the long terminal repeat of human T-lymphotropic virus type I.

ABSTRACT: The proto-oncogene c-myb encodes a nuclear transcription factor that binds to DNA in a sequence-specific manner and activates transcription of several viral and cellular genes. Expression of the c-myb gene is induced in mitogen- and/or antigen-stimulated T lymphocytes, which are also the preferential target cells of human T-lymphotropic virus type I (HTLV-I) in vivo and in vitro. We report here that Myb binds to the HTLV-I long terminal repeat (LTR) in four different regions in a sequence-specific manner. Electrophoretic mobility shift assay using labeled LTR fragments as well as labeled double-stranded oligonucleotides show that there are two high-affinity and two low-affinity Myb-binding sites present in the HTLV-I LTR. DNase I footprinting analysis and oligonucleotide competition experiments indicate that this binding is sequence specific. Cotransfection experiments in HeLa cells, using a Myb expression vector and chloramphenicol acetyltransferase reporter gene linked to the HTLV-I LTR, show that Myb activates HTLV-I LTR-mediated transcription by a factor of four-to sixfold. Thus, in HTLV-I-infected T cells, Myb protein binding to the HTLV-I LTR may constitute one of the signal that regulate HTLV-I transcription in vivo.

SUBMITTER: Dasgupta P 

PROVIDER: S-EPMC238284 | BioStudies | 1992-01-01T00:00:00Z


REPOSITORIES: biostudies

Similar Datasets

2006-01-01 | S-EPMC1617244 | BioStudies
2017-01-01 | S-EPMC5335701 | BioStudies
2020-01-01 | S-EPMC7108837 | BioStudies
2003-01-01 | S-EPMC224568 | BioStudies
2008-01-01 | S-EPMC2293026 | BioStudies
2009-01-01 | S-EPMC2647524 | BioStudies
1000-01-01 | S-EPMC191787 | BioStudies
2006-01-01 | S-EPMC3044894 | BioStudies
2012-01-01 | S-EPMC3373628 | BioStudies
1998-01-01 | S-EPMC109622 | BioStudies