Dataset Information


Super-resolution microscopy reveals that mammalian mitochondrial nucleoids have a uniform size and frequently contain a single copy of mtDNA.

ABSTRACT: Mammalian mtDNA is packaged in DNA-protein complexes denoted mitochondrial nucleoids. The organization of the nucleoid is a very fundamental question in mitochondrial biology and will determine tissue segregation and transmission of mtDNA. We have used a combination of stimulated emission depletion microscopy, enabling a resolution well below the diffraction barrier, and molecular biology to study nucleoids in a panel of mammalian tissue culture cells. We report that the nucleoids labeled with antibodies against DNA, mitochondrial transcription factor A (TFAM), or incorporated BrdU, have a defined, uniform mean size of ?100 nm in mammals. Interestingly, the nucleoid frequently contains only a single copy of mtDNA (average ?1.4 mtDNA molecules per nucleoid). Furthermore, we show by molecular modeling and volume calculations that TFAM is a main constituent of the nucleoid, besides mtDNA. These fundamental insights into the organization of mtDNA have broad implications for understanding mitochondrial dysfunction in disease and aging.


PROVIDER: S-EPMC3158146 | BioStudies | 2011-01-01

REPOSITORIES: biostudies

Similar Datasets

2015-01-01 | S-EPMC4568684 | BioStudies
1000-01-01 | S-EPMC5595809 | BioStudies
2016-01-01 | S-EPMC4854735 | BioStudies
2017-01-01 | S-EPMC5489437 | BioStudies
2016-04-07 | E-GEOD-78243 | ArrayExpress
2011-01-01 | S-EPMC3233019 | BioStudies
2008-01-01 | S-EPMC2442202 | BioStudies
1000-01-01 | S-EPMC4187616 | BioStudies
2007-01-01 | S-EPMC1951767 | BioStudies
1000-01-01 | S-EPMC5728373 | BioStudies