Unknown

Dataset Information

0

5-(4-Hydroxy-2,3,5-trimethylbenzylidene) thiazolidine-2,4-dione attenuates atherosclerosis possibly by reducing monocyte recruitment to the lesion.


ABSTRACT: A variety of benzylidenethiazole analogs have been demonstrated to inhibit 5-lipoxygenase (5-LOX). Here we report the anti-atherogenic potential of 5-(4-hydroxy- 2,3,5-trimethylbenzylidene) thiazolidin-2,4-dione (HMB-TZD), a benzylidenethiazole analog, and its potential mechanism of action in LDL receptor-deficient (Ldlr-/-) mice. HMB-TZD Treatment reduced leukotriene B4 (LTB4) production significantly in RAW264.7 macrophages and SVEC4-10 endothelial cells. Macrophages or endothelial cells pre-incubated with HMB-TZD for 2 h and then stimulated with lipopolysaccharide or tumor necrosis factor-alpha (TNF-?) displayed reduced cytokine production. Also, HMB-TZD reduced cell migration and adhesion in accordance with decreased proinflammatory molecule production in vitro and ex vivo. HMB-TZD treatment of 8-week-old male Ldlr-/- mice resulted in significantly reduced atherosclerotic lesions without a change to plasma lipid profiles. Moreover, aortic expression of pro-atherogenic molecules involved in the recruitment of monocytes to the aortic wall, including TNF-? , MCP-1, and VCAM-1, was downregulated. HMB-TZD also reduced macrophage infiltration into atherosclerotic lesions. In conclusion, HMB-TZD ameliorates atherosclerotic lesion formation possibly by reducing the expression of proinflammatory molecules and monocyte/macrophage recruitment to the lesion. These results suggest that HMB-TZD, and benzylidenethiazole analogs in general, may have therapeutic potential as treatments for atherosclerosis.

SUBMITTER: Choi JH 

PROVIDER: S-EPMC3174381 | BioStudies | 2011-01-01

REPOSITORIES: biostudies

Similar Datasets

2016-01-01 | S-EPMC4879071 | BioStudies
2013-01-01 | S-EPMC3646462 | BioStudies
2014-01-01 | S-EPMC4617132 | BioStudies
2013-01-01 | S-EPMC4001700 | BioStudies
2011-01-01 | S-EPMC3141097 | BioStudies
2019-01-01 | S-EPMC6805995 | BioStudies
2013-01-01 | S-EPMC3751695 | BioStudies
2011-01-01 | S-EPMC3098380 | BioStudies
2020-01-01 | S-EPMC7524356 | BioStudies
2011-01-01 | S-EPMC4010081 | BioStudies