Dataset Information


Alu and LINE-1 methylation and lung function in the normative ageing study.

ABSTRACT: To investigate the association between methylation of transposable elements Alu and long-interspersed nuclear elements (LINE-1) and lung function.Cohort study.Outpatient Veterans Administration facilities in greater Boston, Massachusetts, USA.Individuals from the Veterans Administration Normative Aging Study, a longitudinal study of aging in men, evaluated between 1999 and 2007. The majority (97%) were white.Primary predictor was methylation, assessed using PCR-pyrosequencing after bisulphite treatment. Primary outcome was lung function as assessed by spirometry, performed according to American Thoracic Society/European Respiratory Society guidelines at the same visit as the blood draws.In multivariable models adjusted for age, height, body mass index (BMI), pack-years of smoking, current smoking and race, Alu hypomethylation was associated with lower forced expiratory volume in 1 s (FEV(1)) (?=28 ml per 1% change in Alu methylation, p=0.017) and showed a trend towards association with a lower forced vital capacity (FVC) (?=27 ml, p=0.06) and lower FEV(1)/FVC (?=0.3%, p=0.058). In multivariable models adjusted for age, height, BMI, pack-years of smoking, current smoking, per cent lymphocytes, race and baseline lung function, LINE-1 hypomethylation was associated with more rapid decline of FEV(1) (?=6.9 ml/year per 1% change in LINE-1 methylation, p=0.005) and of FVC (?=9.6 ml/year, p=0.002).In multiple regression analysis, Alu hypomethylation was associated with lower lung function, and LINE-1 hypomethylation was associated with more rapid lung function decline in a cohort of older and primarily white men from North America. Future studies should aim to replicate these findings and determine if Alu or LINE-1 hypomethylation may be due to specific and modifiable environmental exposures.


PROVIDER: S-EPMC3488751 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2010-01-01 | S-EPMC2896391 | BioStudies
2012-01-01 | S-EPMC3527213 | BioStudies
1000-01-01 | S-EPMC3480523 | BioStudies
2020-01-01 | S-EPMC7403370 | BioStudies
2011-01-01 | S-EPMC3172890 | BioStudies
2009-01-01 | S-EPMC3035577 | BioStudies
2013-01-01 | S-EPMC4060265 | BioStudies
2012-01-01 | S-EPMC3380861 | BioStudies
2020-01-01 | S-EPMC7465798 | BioStudies
2019-01-01 | S-EPMC6366765 | BioStudies