Unknown

Dataset Information

0

Clathrin is not required for SNX-BAR-retromer-mediated carrier formation.


ABSTRACT: Clathrin has been implicated in retromer-mediated trafficking, but its precise function remains elusive. Given the importance of retromers for efficient endosomal sorting, we have sought to clarify the relationship between clathrin and the SNX-BAR retromer. We find that the retromer SNX-BARs do not interact directly or indirectly with clathrin. In addition, we observe that SNX-BAR-retromer tubules and carriers are not clathrin coated. Furthermore, perturbing clathrin function, by overexpressing a dominant-negative clathrin or through suppression of clathrin expression, has no detectable effect on the frequency of SNX-BAR-retromer tubulation. We propose that SNX-BAR-retromer-mediated membrane deformation and carrier formation does not require clathrin, and hence the role of clathrin in SNX-BAR-retromer function would appear to lie in pre-SNX-BAR-retromer cargo sorting.

SUBMITTER: McGough IJ 

PROVIDER: S-EPMC3603510 | BioStudies | 2013-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5674888 | BioStudies
2020-01-01 | S-EPMC7082075 | BioStudies
1000-01-01 | S-EPMC3941054 | BioStudies
2017-01-01 | S-EPMC5674890 | BioStudies
2014-01-01 | S-EPMC4159646 | BioStudies
2012-01-01 | S-EPMC3512392 | BioStudies
2017-01-01 | S-EPMC5262529 | BioStudies
2020-01-01 | S-EPMC7083883 | BioStudies
2009-01-01 | S-EPMC2714578 | BioStudies
1000-01-01 | S-EPMC2776105 | BioStudies