Unknown

Dataset Information

0

Morphological transformations in a dually thermoresponsive coil-rod-coil bioconjugate.


ABSTRACT: We report the conformational and assembly behavior of a thermoresponsive triblock biohybrid conjugate under aqueous conditions. The triblock comprises of poly(diethylene glycol methyl ether methacrylate) (PDEGMEMA) conjugated to the ends of a triple-helix forming collagen-like peptide. The circular dichroism (CD) experiment confirms the ability of the collagen-like peptide middle block to assemble as a triple helix in the hybrid conjugate. Above the LCST (~35 °C), the collapse of the thermoresponsive PDEGMEMA polymer at the ends of the peptide domain resulted in a concomitant increase in the conformational stability of the peptide domain towards thermal denaturation. Upon cooling back, the kinetic conformational refolding behavior was still observed for the peptide domain in the hybrid conjugate. Static light scattering (SLS) experiments suggested the formation of supramolecular structures upon increasing solution temperatures to above the LCST. The scattering intensity increased with increasing temperature, until at 75 °C then it was found to decrease. Cryogenic scanning electron microscopy and regular transmission electron microscopy suggested the formation of spherical aggregates that increased in size with increasing temperature up to 65 °C and a morphological transformation into fibrils was also observed at 75 °C. The synergistic effect of dual thermoresponsive behavior from the peptide and the polymer block in the triblock hybrid is suggested for the observed conformational and assembly behaviors.

SUBMITTER: Krishna OD 

PROVIDER: S-EPMC3677730 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6787895 | BioStudies
1000-01-01 | S-EPMC4562312 | BioStudies
2013-01-01 | S-EPMC4256073 | BioStudies
2019-01-01 | S-EPMC6764023 | BioStudies
2017-01-01 | S-EPMC6414999 | BioStudies
2017-01-01 | S-EPMC6318582 | BioStudies
2020-01-01 | S-EPMC7589527 | BioStudies
2013-01-01 | S-EPMC3593141 | BioStudies
2020-01-01 | S-EPMC7330502 | BioStudies
2019-01-01 | S-EPMC6669878 | BioStudies