Unknown

Dataset Information

0

Epitope mapping of M36, a human antibody domain with potent and broad HIV-1 inhibitory activity.


ABSTRACT: M36 is the first member of a novel class of potent HIV-1 entry inhibitors based on human engineered antibody domains (eAds). It exhibits broad inhibitory activity suggesting that its CD4-induced epitope is highly conserved. Here, we describe fine mapping of its epitope by using several approaches. First, a panel of mimotopes was affinity-selected from a random peptide library and potential m36-binding residues were computationally predicted. Second, homology modeling of m36 and molecular docking of m36 onto gp120 revealed potentially important residues in gp120-m36 interactions. Third, the predicted contact residues were verified by site-directed mutagenesis. Taken together, m36 epitope comprising three discontinuous sites including six key gp120 residues (Site C1: Thr123 and Pro124; Site C3: Glu370 and Ile371; Site C4: Met426 and Trp427) were identified. In the 3D structure of gp120, the sites C1 and C4 are located in the bridging sheet and the site C3 is within the ?15-?3 excursion, which play essential roles for the receptor- and coreceptor-binding and are major targets of neutralizing antibodies. Based on these results we propose a precise localization of the m36 epitope and suggest a mechanism of its broad inhibitory activity which could help in the development of novel HIV-1 therapeutics based on eAds.

SUBMITTER: Wan C 

PROVIDER: S-EPMC3679054 | BioStudies | 2013-01-01

REPOSITORIES: biostudies

Similar Datasets

1993-01-01 | S-EPMC1132196 | BioStudies
2017-01-01 | S-EPMC5609902 | BioStudies
2018-01-01 | S-EPMC5841823 | BioStudies
2012-01-01 | S-EPMC3476288 | BioStudies
1000-01-01 | S-EPMC54299 | BioStudies
2013-01-01 | S-EPMC3653109 | BioStudies
1000-01-01 | S-EPMC236373 | BioStudies
1000-01-01 | S-EPMC2579388 | BioStudies
1997-01-01 | S-EPMC19795 | BioStudies
2010-01-01 | S-EPMC3008351 | BioStudies