Dataset Information


Changes in the NS1 gene of avian influenza viruses isolated in Thailand affect expression of type I interferon in primary chicken embryonic fibroblast cells.

ABSTRACT: The non-structural protein 1 (NS1) of avian influenza virus was defined as one of the virulent factors. To understand the effect of NS1 protein of influenza virus H5N1 isolated in Thailand on type I (α/β) interferon (IFN) synthesis, five reverse genetic viruses were constructed and used as models. The viruses were generated using NS genomic segment from A/Peurto Rico/8/1934 (H1N1) and four avian influenza viruses isolated from the first outbreak in Thailand. All the viruses have the rest of the genome from A/Peurto Rico/8/1934 (H1N1). The constructed viruses were named (1) NS1 PR8/34, (2) NS1 wild type, (3) NS1 L15FD53G, (4) NS1 N171I and (5) NS1 E71K, respectively. The type I (α/β) IFN gene expression in control and infected primary chicken embryonic fibroblast cells were analyzed by quantitative polymerase chain reaction. The results show that the inhibition of IFN-β gene expression by NS1 wild type infected cells is stronger than NS1 N171I, NS1 E71K, NS1 PR8/34 and NS1 L15FD53G, respectively. The data suggest that the difference of amino acid sequence of NS1 protein contributes to the IFN-β antagonist. In contrast, the difference of the NS1 protein does not influence in the IFN-α antagonistic activity.

SUBMITTER: Thepmalee C 

PROVIDER: S-EPMC3832696 | BioStudies | 2013-01-01


REPOSITORIES: biostudies

Similar Datasets

2018-01-01 | S-EPMC5972909 | BioStudies
2014-01-01 | S-EPMC4178899 | BioStudies
2011-01-01 | S-EPMC3111539 | BioStudies
2017-01-01 | S-EPMC5331824 | BioStudies
2013-01-01 | S-EPMC3648185 | BioStudies
2010-01-01 | S-EPMC2898259 | BioStudies
2011-01-01 | S-EPMC3100292 | BioStudies
2013-01-01 | S-EPMC3679156 | BioStudies
2012-01-01 | S-EPMC3341361 | BioStudies
2020-01-01 | S-EPMC7232376 | BioStudies