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Elevated Genome-Wide Instability in Yeast Mutants Lacking RNase H Activity.


ABSTRACT: Two types of RNA:DNA associations can lead to genome instability: the formation of R-loops during transcription and the incorporation of ribonucleotide monophosphates (rNMPs) into DNA during replication. Both ribonuclease (RNase) H1 and RNase H2 degrade the RNA component of R-loops, whereas only RNase H2 can remove one or a few rNMPs from DNA. We performed high-resolution mapping of mitotic recombination events throughout the yeast genome in diploid strains of Saccharomyces cerevisiae lacking RNase H1 (rnh1?), RNase H2 (rnh201?), or both RNase H1 and RNase H2 (rnh1? rnh201?). We found little effect on recombination in the rnh1? strain, but elevated recombination in both the rnh201? and the double-mutant strains; levels of recombination in the double mutant were ?50% higher than in the rnh201 single-mutant strain. An rnh201? mutant that additionally contained a mutation that reduces rNMP incorporation by DNA polymerase ? (pol2-M644L) had a level of instability similar to that observed in the presence of wild-type Pol ?. This result suggests that the elevated recombination observed in the absence of only RNase H2 is primarily a consequence of R-loops rather than misincorporated rNMPs.

SUBMITTER: O'Connell K 

PROVIDER: S-EPMC4649664 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

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