Unknown

Dataset Information

0

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection.


ABSTRACT: A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68 nm) and selective ratiometric signal upon Zn(2+) binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn(2+). Mito-MPVQ also exhibited large two-photon absorption cross sections (150 GM) at nearly 720 nm and insensitivity to pH within the biologically relevant pH range. Cell imaging indicated that Mito-MPVQ could efficiently located in mitochondria and monitor mitochondrial Zn(2+) under two-photon excitation with low cytotoxicity.

SUBMITTER: Ning P 

PROVIDER: S-EPMC4673014 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

2015-01-01 | S-EPMC5490428 | BioStudies
1000-01-01 | S-EPMC6273910 | BioStudies
2012-01-01 | S-EPMC3477250 | BioStudies
2019-01-01 | S-EPMC6945768 | BioStudies
2015-01-01 | S-EPMC4612371 | BioStudies
1000-01-01 | S-EPMC5953010 | BioStudies
2011-01-01 | S-EPMC3126897 | BioStudies
2018-01-01 | S-EPMC6057613 | BioStudies
2013-01-01 | S-EPMC3658736 | BioStudies
2012-01-01 | S-EPMC4582792 | BioStudies