Unknown

Dataset Information

0

Spatial and Temporal Changes in the Broiler Chicken Cecal and Fecal Microbiomes and Correlations of Bacterial Taxa with Cytokine Gene Expression.


ABSTRACT: To better understand the ecology of the poultry gastrointestinal (GI) microbiome and its interactions with the host, we compared GI bacterial communities by sample type (fecal or cecal), time (1, 3, and 6?weeks posthatch), and experimental pen (1, 2, 3, or 4), and measured cecal mRNA transcription of the cytokines IL18, IL1?, and IL6, IL10, and TGF-?4. The microbiome was characterized by sequencing of 16S rRNA gene amplicons, and cytokine gene expression was measured by a panel of quantitative-PCR assays targeting mRNAs. Significant differences were observed in the microbiome by GI location (fecal versus cecal) and bird age as determined by permutational MANOVA and UniFrac phylogenetic hypothesis tests. At 1-week posthatch, bacterial genera significantly over-represented in fecal versus cecal samples included Gallibacterium and Lactobacillus, while the genus Bacteroides was significantly more abundant in the cecum. By 6-week posthatch, Clostridium and Caloramator (also a Clostridiales) sequence types had increased significantly in the cecum and Lactobacillus remained over-represented in fecal samples. In the ceca, the relative abundance of sequences classified as Clostridium increased by ca. 10-fold each sampling period from 0.1% at 1?week to 1% at 3?week and 18% at 6?week. Increasing community complexity through time were observed in increased taxonomic richness and diversity. IL18 and IL1? significantly (p?

SUBMITTER: Oakley BB 

PROVIDER: S-EPMC4759570 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5207659 | BioStudies
2018-01-01 | S-EPMC6125325 | BioStudies
2008-01-01 | S-EPMC2492807 | BioStudies
2019-01-01 | S-EPMC6839838 | BioStudies
2015-01-01 | S-EPMC4401635 | BioStudies
2019-01-01 | S-EPMC6449842 | BioStudies
2020-01-01 | S-EPMC7289591 | BioStudies
| S-EPMC6037205 | BioStudies
2017-01-01 | S-EPMC5640251 | BioStudies
2002-01-01 | S-EPMC126540 | BioStudies