Dataset Information


Dynamic Imaging Reveals Coordinate Effects of Cyclic Stretch and Substrate Stiffness on Endothelial Integrity.

ABSTRACT: We describe an equibiaxial cell stretcher and hybrid, elastic membrane platform designed for dynamic imaging of cells on substrates with physiological stiffness undergoing cyclic stretch. Studies enabled by this device revealed that both substrate stiffness and cyclic stretch coordinately protect pulmonary endothelial monolayers against thrombin-induced disruption. The fluorescence imaging possible with the designed hybrid membranes further revealed similarities and differences in actin and cell dynamics during monolayer recovery. The improved live-cell imaging capabilities of this platform, when used in conjunction with fluorescent probes, will have broad applications for investigations of the impact of biochemical stimuli and mechanotransduction mechanisms on mechanically perturbed tissues.


PROVIDER: S-EPMC5114158 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

2015-01-01 | S-EPMC4372154 | BioStudies
2007-01-01 | S-EPMC2583333 | BioStudies
2016-01-01 | S-EPMC4850240 | BioStudies
2018-01-01 | S-EPMC5798351 | BioStudies
2014-01-01 | S-EPMC3963186 | BioStudies
2020-01-22 | GSE120676 | GEO
2004-11-04 | GSE1595 | GEO
2018-03-17 | GSE107551 | GEO
2016-01-01 | S-EPMC4748238 | BioStudies
2017-01-01 | S-EPMC5674939 | BioStudies