Unknown

Dataset Information

0

Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis.


ABSTRACT: Lariat RNAs formed as by-products of splicing are quickly degraded by the RNA debranching enzyme 1 (DBR1), leading to their turnover. Null dbr1 mutants in both animals and plants are embryo lethal, but the mechanism underlying the lethality remains unclear. Here we characterized a weak mutant allele of DBR1 in Arabidopsis, dbr1-2, and showed that a global increase in lariat RNAs was unexpectedly accompanied by a genome-wide reduction in miRNA accumulation. The dbr1-2 mutation had no effects on expression of miRNA biogenesis genes or primary miRNAs (pri-miRNAs), but the association of pri-miRNAs with the DCL1/HYL1 dicing complex was impaired. Lariat RNAs were associated with the DCL1/HYL1 dicing complex in vivo and competitively inhibited the binding of HYL1 with pri-miRNA. Consistent with the impacts of lariat RNAs on miRNA biogenesis, over-expression of lariat RNAs reduced miRNA accumulation. Lariat RNAs localized in nuclear bodies, and partially co-localize with HYL1, and both DCL1 and HYL1 were mis-localized in dbr1-2. Together with our findings that nearly four hundred lariat RNAs exist in wild type plants and that these lariat RNAs also associate with the DCL1/HYL1 dicing complex in vivo, we thus propose that lariat RNAs, as decoys, inhibit miRNA processing, suggesting a hitherto unknown layer of regulation in miRNA biogenesis.

SUBMITTER: Li Z 

PROVIDER: S-EPMC5147768 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3641593 | BioStudies
2012-10-27 | E-GEOD-41843 | BioStudies
2007-01-01 | S-EPMC1950788 | BioStudies
1000-01-01 | S-EPMC4231765 | BioStudies
2012-01-01 | S-EPMC3412041 | BioStudies
2012-10-27 | E-GEOD-41843 | ArrayExpress
2019-01-01 | S-EPMC6811466 | BioStudies
1000-01-01 | S-EPMC2481344 | BioStudies
2020-01-01 | S-EPMC7474595 | BioStudies
1000-01-01 | S-EPMC3561960 | BioStudies