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Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal.


ABSTRACT: We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.

SUBMITTER: Li C 

PROVIDER: S-EPMC5382767 | BioStudies | 2017-01-01T00:00:00Z

REPOSITORIES: biostudies

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