Unknown

Dataset Information

0

Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis.


ABSTRACT: Activin B, a homodimer of inhibin beta b (INHBB), is a multifunctional cytokine belonging to the transforming growth factor-β (TGF-β) family. However, the molecular functions and clinical relevance of activin B have not been determined in oral cancer. We investigated the critical roles of activin B in oral squamous cell carcinoma (OSCC). We performed quantitative reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemistry to study INHBB expression in OSCC-derived cell lines and OSCC clinical samples. The INHBB expression levels were significantly (P < 0.05) overexpressed in OSCCs compared to normal counterparts in vitro and in vivo. Activin B-positivity in OSCC cases was significantly (P < 0.05) correlated with regional lymph node metastasis. The INHBB knockdown (shINHBB) cells promoted cellular adhesion and suppression of cellular invasiveness and migration. After treatment of shINHBB cells with activin B, those activities were restored similar to the shMock cells. In the processes of invasiveness and metastasis, the cells cause epithelial-mesenchymal transition (EMT). TGF-β and its family members are promoters of the EMT process. To investigate whether activin B is related to EMT, we examined the expressions of EMT-related genes and found that INHBB was related closely to EMT. Our results suggested for the first time that activin B indicates tumoral metastasis in OSCCs and might be a useful biomarker for OSCC metastasis.

SUBMITTER: Kita A 

PROVIDER: S-EPMC5559965 | BioStudies | 2017-01-01

SECONDARY ACCESSION(S): 10.7150/jca.18714

REPOSITORIES: biostudies

altmetric image

Publications

Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis.

Kita Akihiro A   Kasamatsu Atsushi A   Nakashima Dai D   Endo-Sakamoto Yosuke Y   Ishida Sho S   Shimizu Toshihiro T   Kimura Yasushi Y   Miyamoto Isao I   Yoshimura Shusaku S   Shiiba Masashi M   Tanzawa Hideki H   Uzawa Katsuhiro K  

Journal of Cancer 20170705 11


Activin B, a homodimer of inhibin beta b (INHBB), is a multifunctional cytokine belonging to the transforming growth factor-β (TGF-β) family. However, the molecular functions and clinical relevance of activin B have not been determined in oral cancer. We investigated the critical roles of activin B in oral squamous cell carcinoma (OSCC). We performed quantitative reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemistry to study INHBB expression in OSCC-derived  ...[more]

Similar Datasets

2015-01-01 | S-EPMC4552554 | BioStudies
1000-01-01 | S-EPMC4258025 | BioStudies
2015-01-01 | S-EPMC4372572 | BioStudies
2014-01-01 | S-EPMC3903644 | BioStudies
1000-01-01 | S-EPMC3731802 | BioStudies
2018-01-01 | S-EPMC5958702 | BioStudies
2010-01-01 | S-EPMC2949797 | BioStudies
1000-01-01 | S-EPMC1073677 | BioStudies
2006-01-01 | S-EPMC2064178 | BioStudies
2017-01-01 | S-EPMC5322526 | BioStudies