Dataset Information


OsMTP11 is localised at the Golgi and contributes to Mn tolerance.

ABSTRACT: Membrane transporters play a key role in obtaining sufficient quantities of manganese (Mn) but also in protecting against Mn toxicity. We have characterized OsMTP11, a member of the Cation Diffusion Facilitator/Metal Tolerance Protein (CDF/MTP) family of metal cation transporters in Oryza sativa. We demonstrate that OsMTP11 functions in alleviating Mn toxicity as its expression can rescue the Mn-sensitive phenotype of the Arabidopsis mtp11-3 knockout mutant. When expressed stably in Arabidopsis and transiently in rice and tobacco, it localises to the Golgi. OsMTP11 partially rescues the Mn-hypersensitivity of the pmr1 yeast mutant but only slightly alleviates the Zn sensitivity of the zrc1 cot1 yeast mutant. Overall, these results suggest that OsMTP11 predominantly functions as a Mn-transporting CDF with lower affinity for Zn. Site-directed mutagenesis studies revealed four substitutions in OsMTP11 that appear to alter its transport activity. OsMTP11 harbouring a substitution of leucine 150 to a serine fully rescued pmr1 Mn-sensitivity at all concentrations tested. The other substitutions, including those at conserved DxxxD domains, reduced complementation of pmr1 to different levels. This indicates their importance for OsMTP11 function and is a starting point for refining transporter activity/specificity.


PROVIDER: S-EPMC5681648 | BioStudies | 2017-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3697945 | BioStudies
2001-01-01 | S-EPMC55566 | BioStudies
2019-01-01 | S-EPMC6440370 | BioStudies
1000-01-01 | S-EPMC4800876 | BioStudies
2008-01-01 | S-EPMC2417167 | BioStudies
1000-01-01 | S-EPMC1868760 | BioStudies
2019-01-01 | S-EPMC6305187 | BioStudies
1000-01-01 | S-EPMC2632156 | BioStudies
2009-01-01 | S-EPMC4378827 | BioStudies
2012-01-01 | S-EPMC3270973 | BioStudies