Unknown

Dataset Information

0

The axonal transport motor kinesin-2 navigates microtubule obstacles via protofilament switching.


ABSTRACT: Axonal transport involves kinesin motors trafficking cargo along microtubules that are rich in microtubule-associated proteins (MAPs). Much attention has focused on the behavior of kinesin-1 in the presence of MAPs, which has overshadowed understanding the contribution of other kinesins such as kinesin-2 in axonal transport. We have previously shown that, unlike kinesin-1, kinesin-2 in vitro motility is insensitive to the neuronal MAP Tau. However, the mechanism by which kinesin-2 efficiently navigates Tau on the microtubule surface is unknown. We hypothesized that mammalian kinesin-2 side-steps to adjacent protofilaments to maneuver around MAPs. To test this, we used single-molecule imaging to track the characteristic run length and protofilament switching behavior of kinesin-1 and kinesin-2 motors in the absence and presence of 2 different microtubule obstacles. Under all conditions tested, kinesin-2 switched protofilaments more frequently than kinesin-1. Using computational modeling that recapitulates run length and switching frequencies in the presence of varying roadblock densities, we conclude that kinesin-2 switches protofilaments to navigate around microtubule obstacles. Elucidating the kinesin-2 mechanism of navigation on the crowded microtubule surface provides a refined view of its contribution in facilitating axonal transport.

SUBMITTER: Hoeprich GJ 

PROVIDER: S-EPMC5687255 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC4008791 | BioStudies
1000-01-01 | S-EPMC4423065 | BioStudies
2018-01-01 | S-EPMC5783771 | BioStudies
2016-01-01 | S-EPMC4726810 | BioStudies
2004-01-01 | S-EPMC2172520 | BioStudies
2019-01-01 | S-EPMC6783262 | BioStudies
2008-01-01 | S-EPMC2866193 | BioStudies
2018-01-01 | S-EPMC5902456 | BioStudies
2014-01-01 | S-EPMC4248269 | BioStudies
2019-07-08 | S-BSST266 | BioStudies