Unknown

Dataset Information

0

Dynamic Combinatorial Chemistry to Identify Binders of ThiT, an S-Component of the Energy-Coupling Factor Transporter for Thiamine.


ABSTRACT: We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S-component of the energy-coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre-equilibrated dynamic combinatorial library (DCL) and saturation-transfer difference (STD) NMR spectroscopy to identify ligands of ThiT. This is the first report in which DCC is used for fragment growing to an ill-defined pocket, and one of the first reports for its application with an integral membrane protein as target.

SUBMITTER: Monjas L 

PROVIDER: S-EPMC5698757 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

Similar Datasets

2021-01-01 | S-EPMC7818428 | BioStudies
1000-01-01 | S-EPMC5947633 | BioStudies
2019-01-01 | S-EPMC6595003 | BioStudies
2016-01-01 | S-EPMC5477050 | BioStudies
2009-01-01 | S-EPMC4458376 | BioStudies
2019-01-01 | S-EPMC6600254 | BioStudies
2012-01-01 | S-EPMC3652255 | BioStudies
2018-01-01 | S-EPMC6282583 | BioStudies
2016-01-01 | S-EPMC5113778 | BioStudies
2019-01-01 | S-EPMC6979337 | BioStudies