Unknown

Dataset Information

0

Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.


ABSTRACT: Platelet integrity and function critically depend on lipid composition. However, the lipid inventory in platelets was hitherto not quantified. Here, we examined the lipidome of murine platelets using lipid-category tailored protocols on a quantitative lipidomics platform. We could show that the platelet lipidome comprises almost 400 lipid species and covers a concentration range of 7 orders of magnitude. A systematic comparison of the lipidomics network in resting and activated murine platelets, validated in human platelets, revealed that <20% of the platelet lipidome is changed upon activation, involving mainly lipids containing arachidonic acid. Sphingomyelin phosphodiesterase-1 (Smpd1) deficiency resulted in a very specific modulation of the platelet lipidome with an order of magnitude upregulation of lysosphingomyelin (SPC), and subsequent modification of platelet activation and thrombus formation. In conclusion, this first comprehensive quantitative lipidomic analysis of platelets sheds light on novel mechanisms important for platelet function, and has therefore the potential to open novel diagnostic and therapeutic opportunities.

SUBMITTER: Peng B 

PROVIDER: S-EPMC6137561 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC2650121 | BioStudies
2020-01-01 | S-EPMC7722935 | BioStudies
1000-01-01 | S-EPMC4873619 | BioStudies
2012-01-01 | S-EPMC3329542 | BioStudies
1000-01-01 | S-EPMC4941562 | BioStudies
2016-01-01 | S-EPMC4906355 | BioStudies
2019-01-01 | S-EPMC6695943 | BioStudies
2017-01-01 | S-EPMC5408749 | BioStudies
2020-01-01 | S-EPMC7449968 | BioStudies
2018-01-01 | S-EPMC6128932 | BioStudies