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Protein Phosphatase 1? and Cofilin Regulate Nuclear Translocation of NF-?B and Promote Expression of the Anti-Inflammatory Cytokine Interleukin-10 by T Cells.

ABSTRACT: While several protein serine/threonine kinases control cytokine production by T cells, the roles of serine/threonine phosphatases are largely unexplored. Here, we analyzed the involvement of protein phosphatase 1? (PP1?) in cytokine synthesis following costimulation of primary human T cells. Small interfering RNA (siRNA)-mediated knockdown of PP1? (PP1KD) or expression of a dominant negative PP1? (D95N-PP1) drastically diminished interleukin-10 (IL-10) production. Focusing on a key transcriptional activator of human IL-10, we demonstrate that nuclear translocation of NF-?B was significantly inhibited in PP1KD or D95N-PP1 cells. Interestingly, knockdown of cofilin, a known substrate of PP1 containing a nuclear localization signal, also prevented nuclear accumulation of NF-?B. Expression of a constitutively active nonphosphorylatable S3A-cofilin in D95N-PP1 cells restored nuclear translocation of NF-?B and IL-10 expression. Subpopulation analysis revealed that defective nuclear translocation of NF-?B was most prominent in CD4+ CD45RA- CXCR3- T cells that included IL-10-producing TH2 cells. Together these findings reveal novel functions for PP1? and its substrate cofilin in T cells namely the regulation of the nuclear translocation of NF-?B and promotion of IL-10 production. These data suggest that stimulation of PP1? could limit the overwhelming immune responses seen in chronic inflammatory diseases.

PROVIDER: S-EPMC6206454 | BioStudies |

REPOSITORIES: biostudies

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