Dataset Information


Minocycline microspheres did not significantly improve outcomes after collagenase injection of tendon.

ABSTRACT: Background:Tetracycline antibiotics inhibit matrix metalloproteinases and pro-inflammatory cytokines implicated in the pathogenesis of tendinopathy, while microsphere formulations allow sustained release of drug contents. The purpose of this study was to evaluate the ability of a local minocycline microsphere injection to restore normal tendon properties in a rat model of collagenase-induced patellar tendinopathy. Methods:A total of 22 rats were randomly assigned to the control (n?=?11) or minocycline (n?=?11) group and received bilateral patellar tendon injections of collagenase. After 7 days, the minocycline group received the minocycline microsphere treatment and the control group received phosphate buffered solution. Pain was assessed via activity monitors and Von Frey filament testing. At 4 weeks post-collagenase injections, animals were euthanized. Results:Cage crossings significantly decreased among all rats 2-3 days following each injection period, however, tactile allodynia measures did not reflect this injury response. Biomechanical properties, interleukin-1 beta levels, and glycosaminoglycan content did not differ between groups. While not statistically significant, levels of leukotriene B4 were lower in the minocycline group compared to controls (p?=?0.061), suggesting a trend. Conclusions:Our study further characterizes the collagenase model of tendinopathy by demonstrating no evidence of central sensitization with collagenase-induced injury. We found no adverse effect of intratendinous injections of minocycline-loaded poly-lactic-co-glycolic acid microspheres, although no therapeutic effect was observed. Future studies involving a more substantial tendon injury with a greater inflammatory component may be necessary to more thoroughly evaluate the effects of minocycline on tendon pathology.


PROVIDER: S-EPMC6806656 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC4547110 | BioStudies
2018-01-01 | S-EPMC5811024 | BioStudies
2013-01-01 | S-EPMC3765363 | BioStudies
2020-01-01 | S-EPMC7358637 | BioStudies
1000-01-01 | S-EPMC3558233 | BioStudies
2014-01-01 | S-EPMC4001357 | BioStudies
2008-01-01 | S-EPMC2505252 | BioStudies
2017-01-01 | S-EPMC5549401 | BioStudies
| GSE80396 | GEO
2020-01-01 | S-EPMC7045155 | BioStudies