Dataset Information


Lipid is heterogeneously distributed in muscle and associates with low radiodensity in cancer patients.

ABSTRACT: BACKGROUND:Low muscle radiodensity is associated with mortality in a variety of cancer types. Biochemical and morphological correlates are unknown. We aimed to evaluate triglyceride (TG) content and location as a function of computed tomography (CT)-derived measures of skeletal muscle radiodensity in cancer patients. METHODS:Rectus abdominis (RA) biopsies were collected during cancer surgery from 75 patients diagnosed with cancer. Thin-layer chromatography and gas chromatography were used for quantification of TG content of the muscle. Axial CT images of lumbar vertebra were used to measure muscle radiodensity. Oil Red O staining was used to determine the location of neutral lipids in frozen muscle sections. RESULTS:There was wide variation in RA radiodensity in repeated measures (CV% ranged from 3 to 55% based on 10 serial images) as well as within one slice (CV% ranged from 6 to 61% based on 10 subregions). RA radiodensity and total lumbar muscle radiodensity were inversely associated with TG content of RA (r = -0.396, P < 0.001, and r = -0.355, P = 0.002, respectively). Of the total percentage area of muscle staining positive for neutral lipid, 54 ± 17% was present as extramyocellular lipids (range 23.5-77.8%) and 46 ± 17% (range 22.2-76.5%) present as intramyocellular lipid droplets. CONCLUSIONS:Repeated measures revealed wide variation in radiodensity of RA muscle, both vertically and horizontally. Low muscle radiodensity reflects high level of TG in patients with cancer. Non-uniform distribution of intramyocellular and extramyocellular lipids was evident using light microscopy. These results warrant investigation of mechanisms resulting in lipid deposition in muscles of cancer patients.


PROVIDER: S-EPMC7296261 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC5714998 | BioStudies
2018-01-01 | S-EPMC5951728 | BioStudies
2016-01-01 | S-EPMC5014190 | BioStudies
2020-01-01 | S-EPMC7497241 | BioStudies
2014-01-01 | S-EPMC4184063 | BioStudies
2017-01-01 | S-EPMC5725420 | BioStudies
2020-01-01 | S-EPMC6995288 | BioStudies
2019-01-01 | S-EPMC6407435 | BioStudies
2012-01-01 | S-EPMC3357610 | BioStudies
2015-01-01 | S-EPMC4692267 | BioStudies