Unknown

Dataset Information

0

FAM64A: A Novel Oncogenic Target of Lung Adenocarcinoma Regulated by Both Strands of miR-99a (miR-99a-5p and miR-99a-3p).


ABSTRACT: Lung adenocarcinoma (LUAD) is the most aggressive cancer and the prognosis of these patients is unfavorable. We revealed that the expression levels of both strands of miR-99a (miR-99a-5p and miR-99a-3p) were significantly suppressed in several cancer tissues. Analyses of large The Cancer Genome Atlas (TCGA) datasets showed that reduced miR-99a-5p or miR-99a-3p expression is associated with worse prognoses in LUAD patients (disease-free survival (DFS): p = 0.1264 and 0.0316; overall survival (OS): p = 0.0176 and 0.0756, respectively). Ectopic expression of these miRNAs attenuated LUAD cell proliferation, suggesting their tumor-suppressive roles. Our in silico analysis revealed 23 putative target genes of pre-miR-99a in LUAD cells. Among these targets, high expressions of 19 genes were associated with worse prognoses in LUAD patients (OS: p < 0.05). Notably, FAM64A was regulated by both miR-99a-5p and miR-99a-3p in LUAD cells, and its aberrant expression was significantly associated with poor prognosis in LUAD patients (OS: p = 0.0175; DFS: p = 0.0276). FAM64A knockdown using siRNAs suggested that elevated FAM64A expression contributes to cancer progression. Aberrant FAM64A expression was detected in LUAD tissues by immunostaining. Taken together, our miRNA-based analysis might be effective for identifying prognostic and therapeutic molecules in LUAD.

SUBMITTER: Mizuno K 

PROVIDER: S-EPMC7564711 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6953126 | BioStudies
2014-01-01 | S-EPMC4167263 | BioStudies
2020-01-01 | S-EPMC7230175 | BioStudies
2018-01-01 | S-EPMC5757495 | BioStudies
2018-01-01 | S-EPMC5943442 | BioStudies
2018-01-01 | S-EPMC6070648 | BioStudies
2019-01-01 | S-EPMC6562801 | BioStudies
2018-01-01 | S-EPMC6217763 | BioStudies
2020-01-01 | S-EPMC7670601 | BioStudies
2018-01-01 | S-EPMC6069561 | BioStudies