BART-seq: cost-effective massively parallel targeted sequencing for genomics and transcriptomics [genotyping]

ABSTRACT: We report the application of a novel workflow, BART-Seq (Barcode Assembly foR Targeted Sequencing), the first highly sensitive, quantitative, and inexpensive technique for enriching selected cohorts of transcripts and genomic regions from thousands of samples in parallel, for next-generation sequencing (NGS). Multiplexing is based on a simple method for producing virtually unlimited matrices of barcoded primer sets. The method provides sample-specific amplicon labels for hundreds-fold enrichment compared to unbiased transcriptomics and genomics approaches. We demonstrated the technique by screening cancer patients for BRCA mutations. We believe that BART-Seq will complement low-sensitivity global NGS approaches, such as droplet-based sequencing, by enabling analysis, validation, and screening of specific processes in biomedical research. Overall design: 10 regions from BRCA1 and BRCA2 genes were co-amplified from genomic DNA samples of 96 breast cancer patients. The amplicons are barcoded using the BART-seq method, and multiplexed for sequencing.

INSTRUMENT(S): Illumina MiSeq (Homo sapiens)

ORGANISM(S): Homo Sapiens

SUBMITTER: Fatma Uzbas  

PROVIDER: GSE107722 | GEO | 2018-10-01

REPOSITORIES: GEO