Genomics

Dataset Information

30

Effect of long and short term nutrition on the response of female Drosophila melanogaster to male sex peptide


ABSTRACT: We used RNAseq to detect differential gene expression in Drosophila melanogaster. We measured gene expression in females response to receipt of male ejaculate sex peptide. We used triple replicated experimental evolution lines in which adults had been selected on food containing 20% or 120% of the normal amount of dietary yeast. These lines had been selected for 51 generations at the time of these experiments. The design of the experiment was fully balanced. We tested the response of high and low food regime females on high and low food diets to matings with control or sex peptide-lacking males. Overall design: The overall experimental design was 3 replicates x 2 experimental evolution regimes (high and low) x 2 immediate diets (high diet and low diet) x 3 treatments (virgin, SP+ or SP0 mated) x 2 body parts = (Head/Thorax and Abdomen). This gave a total of 72 samples for RNAseq. The 100% sugar yeast (SY) diet used was 100 g brewer's yeast, 100 g sucrose, 20 g agar, 30 ml Nipagin (10% w/v solution), 3 ml propionic acid per 1 L water. The high food was as per the 100% diet except that it contained 150g yeast per L, and the low food 20g per L. The flies were maintained at 25 degrees C on a 12h light and 12h dark light regime at 50% relative humidity.

INSTRUMENT(S): Illumina HiSeq 2000 (Drosophila melanogaster)

ORGANISM(S): Drosophila Melanogaster

SUBMITTER: Irina Mohorianu  

PROVIDER: GSE62476 | GEO |

REPOSITORIES: GEO