Transcriptomics,Multiomics

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Single-cell transcriptomics identifies a distinct luminal progenitor cell type in the distal prostate invagination tips


ABSTRACT: The identification of prostate stem/progenitor cells and characterization of the prostate epithelial cell lineage hierarchy are critical for understanding prostate cancer initiation. Here, we characterized 35,129 cells from mouse prostates, and identified a unique luminal cell type (termed type C luminal cell (Luminal-C)) marked by Tacstd2, Ck4 and Psca expression. Luminal-C cells located at the distal prostate invagination tips (termed Dist-Luminal-C) exhibited greater capacity for organoid formation in vitro and prostate epithelial duct regeneration in vivo. Lineage tracing of Luminal-C cells indicated that Dist-Luminal-C cells reconstituted distal prostate luminal lineages through self-renewal and differentiation. Deletion of Pten in Dist-Luminal-C cells resulted in prostatic intraepithelial neoplasia. We further characterized 11,374 human prostate cells and confirmed the existence of h-Luminal-C cells. Our study provides insights into the prostate lineage hierarchy, identifies Dist-Luminal-C cells as the luminal progenitor cell population in invagination tips and suggests one of the potential cellular origins of prostate cancer.

TISSUE(S): Prostate

SUBMITTER: Wangxin,Guo 

PROVIDER: OEX003099 | NODE |

REPOSITORIES: NODE

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Single-cell transcriptomics identifies a distinct luminal progenitor cell type in distal prostate invagination tips.

Guo Wangxin W   Li Lin L   He Juan J   Liu Zhuang Z   Han Ming M   Li Fei F   Xia Xinyi X   Zhang Xiaoyu X   Zhu Yao Y   Wei Yu Y   Li Yunguang Y   Aji Rebiguli R   Dai Hao H   Wei Hui H   Li Chunfeng C   Chen Yu Y   Chen Luonan L   Gao Dong D  

Nature genetics 20200817 9


The identification of prostate stem/progenitor cells and characterization of the prostate epithelial cell lineage hierarchy are critical for understanding prostate cancer initiation. Here, we characterized 35,129 cells from mouse prostates, and identified a unique luminal cell type (termed type C luminal cell (Luminal-C)) marked by Tacstd2, Ck4 and Psca expression. Luminal-C cells located at the distal prostate invagination tips (termed Dist-Luminal-C) exhibited greater capacity for organoid for  ...[more]

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