Project description:To investigate the effect of Burkholderia fungorum (BF) on cholangiocarcinoma cells, we treated the cell lines with bacterial supernatant and performed transcriptome sequencing. By transcriptome sequencing, we quantified the differentially expressed genes in control and experimental groups. The results of GO and KEGG functional enrichment analysis showed that the differentially expressed genes were mainly involved in some biological processes of metabolism and amino acid synthesis. We inferred that the transcriptome characterization can help us understand the relationship between bacterium and tumor cell metabolism.
Project description:In order to understand the consequences of the LPS from different bacterias, we analyzed RNA gene expression profiles in a human myeloid cell line THP-1 cells. RNA-seq transcriptome was analyzed in THP-1 cells treated with LPS from three bacteria B. fungorum, S. marcescens, and P. myrsinacearum.
Project description:[1] Transcription profiling of one Burkholderia cenocepacia clinical isolate, J2315, versus a soil isolate, HI2424, in conditions mimicking CF sputum [2] Transcription profiling of Burkholderia cenocepacia isolates J2315 and HI2424 in media mimicking CF sputum or the soil environment
Project description:Gene expression profiles of human cell (THP-1) lines exposed to a novel Daboiatoxin (DbTx) isolated from Daboia russelli russelli, and specific cytokines and inflammatory pathways involved in acute infection caused by Burkholderia pseudomallei. Keywords: Melioidosis, Burkholderia pseudomallei, Daboiatoxin, Cytokines, Inflammation.