Genomics

Dataset Information

0

Saccharomyces cerevisiae


ABSTRACT: Yeast mRNA decay analysis after addition of 3 & 10 µ/mL thiolutin

ORGANISM(S): Saccharomyces cerevisiae  

PROVIDER: PRJNA101811 | ENA |

REPOSITORIES: ENA

altmetric image

Publications

The transcriptional inhibitor thiolutin blocks mRNA degradation in yeast.

Pelechano Vicent V   Pérez-Ortín José E JE  

Yeast (Chichester, England) 20080201 2


Thiolutin is commonly used as a general inhibitor of transcription in yeast. It has been used to calculate mRNA decay rates by stopping the transcription and then determining the relative abundance of individual mRNAs at different times after inhibition. We report here that thiolutin is also an inhibitor of mRNA degradation, and thus its use can lead to miscalculations of mRNA half-lives. The inhibition of mRNA decay seems to affect the mRNA degradation pathway without impeding poly(A) shortenin  ...[more]

Similar Datasets

| PRJNA98017 | ENA
| PRJNA258654 | ENA
2012-06-17 | E-GEOD-33712 | ArrayExpress
| PRJNA91935 | ENA
2008-10-01 | GSE12221 | GEO
| PRJNA148473 | ENA
2014-02-21 | E-GEOD-55213 | ArrayExpress
| PRJNA93077 | ENA
| GSE33427 | GEO
| PRJNA101417 | ENA