7,12-Dimethylbenz[a]anthracene (DMBA)-induced rat mammary carcinoma is a well-recognized model; however, the genetic alterations during its carcinogenesis have yet to be determined. We used laser capture microdissection to specifically isolate cells from terminal end buds (TEBs), the origin of carcinoma, at 2 weeks after sesame oil treatment (control) or DMBA treatment (DMBA-TEBs), ductal carcinoma in situ (DCIS) and invasive mammary carcinoma (MC). Using an oligonucleotide microarray representi ...[more]
Project description:Analysis of LBNF1 rat testes from controls, containing both somatic and all germ cell types and from irradiated rats in which all cells germ cells except type A spermatgogonia are eliminated. Results provide insight into distinguishing germ and somatic cell genes and identification of somatic cell genes that are upregulated after irradiation. Overall design: We studied 10 Sample replicates of control rat testes and 5 Sample replicates of irradiated rat testes.
Project description:In order to establish a rat embryonic stem cell transcriptome, mRNA from rESC cell line DAc8, the first male germline competent rat ESC line to be described and the first to be used to generate a knockout rat model was characterized using RNA sequencing (RNA-seq) analysis. 2 biological replicates of RRRC#464 DA-EC8/Rrrc cell line were sequenced with 50bp paired end reads using Illumina Hi-Seq 2000
Project description:Inflammation is a key component of pathological angiogenesis. Here we induce cornea neovascularisation using sutures placed into the cornea, and sutures are removed to induce a regression phase. We used whole transcriptome microarray to monitor gene expression profies of several genes Overall design: This was a time course study conprising two arms. In the suture IN arm, sutures were maintained in the cornea after the 0h time point, while suture OUT arm is where both sutures were removed from the cornea at the 0h timpoint. 0h timepoint is when sprouts were 3/4 the distance from the pre-existing limbal vessels towards the sutures. Microarrays were performed at 0h, 24h suture IN and 24h suture OUT. Non sutured eyes from separate animals were used as controls. At each time point except for 0h=3 rats, the rest of the timepoints had four rats each.