Genomics

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Engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus


ABSTRACT: Engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus

PROVIDER: PRJNA283273 | ENA |

REPOSITORIES: ENA

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Successful RNAi applications depend on strategies allowing robust and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. Here, we propose a novel avenue which is integration of a promoterless shmiRNA, i.e. a shRNA embedded in a micro-RNA (miRNA) scaffold, into an engineered genomic miRNA locus. For proof-of-concept, we used TALE or CRISPR/Cas9 nucleases to site-specifically integrate an anti-hepatitis C virus (HCV) shmiRNA into the liver-speci  ...[more]

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