Project description:The goal of this study was to perform RNA-seq expression analysis on Solanum lycopersicum cv. M82 X S. pennellii introgression lines, deriving expression Quantitative Trait Loci which were analyzed together with pre-existing genomic and phenotypic data to define genes and regulatory pathways controlling tomato root development and observed natural variation. We completed the RNAseq expression profiling analysis and developed a tool to display this information graphically in collaboration with Nicholas Provart at the University of Toronto: http://bar.utoronto.ca/efp_tomato/cgi-bin/efpWeb.cgi?dataSource=ILs_Root_Tip_Brady_Lab To identify candidate genes and pathways we focussed on one root growth trait, root growth angle, and identified two statistically significant genomic regions within tomato root growth angle QTL containing two candidate genes that likely control the gravitropic setpoint angle (CDC73 and PAP27), both of which are conserved between Arabidopsis and tomato, and which we tested using transgenic lines of the Arabidopsis orthologs. A possible regulatory role for suberin in root growth angle control was also identified.

2018-01-01 | GSE87162 | GEO

Small RNA profiling Tomato, wild tomato relative and series of introgression lines

Project description:High throughput sequencing was used to investigate the production of small RNAs from cultivated tomato cultivar M82 and its wild relative Solanum pennellii. In order to understand the pattern of inheritance of the samll RNAs, interspecific hybrids (F1 and F2) along with series of introgressed lines comprising precise short genomic regions from S. pennellii in M82 background were used.

2012-05-29 | GSE23562 | GEO

Transcription profiling of Medicago truncatula seeds at 14, 16, 20, 24, and 36 days post pollination

Project description:This experiment investigates a time series of seed development in Medicago truncatula. Time points 14, 16, 20, 24, and 36 days post pollination are hybridized against a 12 days post pollination reference stage.

2007-10-08 | E-MEXP-907 | biostudies-arrayexpress

Small RNA profiling Tomato, wild tomato relative and series of introgression lines

2012-05-29 | E-GEOD-23562 | biostudies-arrayexpress