Klebsiella pneumoniae strain:1219
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA314222 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:hvKP ATCC43816 and its lytic phage H5 were employed as a phage-antibiotic combination model. Based on the comprehensive characterization of phages, including cryo-electron microscopy, we evaluated the synergic effect of H5 on bacterial killing in vitro when combined with multiple antibiotics, and analyzed the advantages of phage-antibiotic combinations from an evolutionary perspective and proposes a novel PAS mechanism by using ceftazidime as an example.
2023-09-14 | GSE237490 | GEO
Project description:OXA-48-carbapenemase producing Enterobacteriaceae. Genome sequencing and assembly
| PRJNA550332 | ENA
Project description:OXA-48 producing Klebsiella pneumoniae, genome sequencing and assembly
| PRJNA419295 | ENA
Project description:OXA-48-carbapenemase producing Enterobacteriaceae. Genome sequencing and assembly
| PRJNA420621 | ENA
Project description:Klebsiella pneumoniae Oxa-48 strain 1191100241 Genome Sequencing Project
| PRJNA71587 | ENA
Project description:K. pneumoniae, a Gram-negative bacterium, is normally associated with pneumonia in patients with weakened immune systems. However, it is also a prevalent nosocomial infectious agent that can be found in infected surgical sites and combat wounds. Many of these clinical strains display multi-drug resistance. We have worked with a clinical strain of K. pneumoniae that was initially isolated from a wound of an injured soldier. This strain demonstrated resistance to many commonly used antibiotics, but sensitivity to carbapenems. This isolate was capable of forming biofilms in vitro, contributing to its increased antibiotic resistance and impaired clearance. We were interested in determining how sublethal concentrations of carbapenem treatment specifically affect K. pneumoniae biofilms both in morphology and genomic expression. Scanning electron microscopy showed striking morphological differences between untreated and treated biofilms, including rounding, blebbing, and dimpling of treated cells. Comparative transcriptome analysis using RNA sequencing technology identified a large number of open reading frames (ORFs) differentially regulated in response to carbapenem treatment at 2 and 24 hours. ORFs upregulated with carbapenem treatment included genes involved in resistance, antiporters, and autoinducers. ORFs downregulated included metal transporters, membrane biosynthesis proteins, and motility proteins. Quantitative real time PCR validated the general trend of some of these differentially regulated ORFs. Treating K. pneumoniae biofilms with sublethal concentrations of carbapenems induced a wide-range of phenotypic and gene expression changes. This study reveals some of the mechanisms underlying how sublethal amounts of carbapenems could affect the overall fitness and pathogenic potential of K. pneumoniae biofilm cells.
2015-01-01 | GSE62045 | GEO
Project description:Emergence of Klebsiella pneumoniae clinical isolates co-producing NDM-5 and OXA-48-like in Nepal
| PRJDB8799 | ENA
Project description:Ceftazidime resistant Klebsiella pneumoniae
| PRJNA577627 | ENA
Project description:Klebsiella pneumoniae strain:1219 Genome sequencing and assembly
| PRJNA412518 | ENA
Project description:Whole genome sequencing of high-risk clones of OXA-48-producing Klebsiella pneumoniae from the veterinary setting in Switzerland
| PRJNA539891 | ENA