Project description:Transcriptional Analysis of Deinococcus radiodurans for Novel sRNAs expression that are Differentially Expressed under Ionizing Radiation
Project description:In Deinococcus radiodurans, a previously unreported special characteristic of DrOxyR (DR0615) is found with only one conserved cysteine. dr0615 gene mutant is hypersensitive to H2O2, but a little to ionizing radiation. Site-directed mutagenesis and subsequent in vivo functional analyses revealed that the conserved cysteine (C210) is necessary for sensing H2O2, but its mutation did not alter the binding characteristics of OxyR on DNA. Under oxidant stress, DrOxyR is oxidized to sulfenic acid form, which can be reduced by reducing reagents. In addition, quantitative real-time PCR and global transcription profile results showed that OxyR is not only a transcriptional activator (e.g., katE, drb0125), but also a transcriptional repressor (e.g., dps, mntH). Transcriptional profiling of comparing wildtype strains with oxyR disruption strains under normal growth conditions. Keywords: Genetic modification
Project description:Even though these results have shown several key proteins played an import role in radiation tolerance mechanism of DR, they were lack of the deep protein coverage, global protein analysis as well as the dynamic changes of DR, which limits further understanding of biological changes in response to ionizing radiation. To the best of our knowledge, the global and specific radiation tolerance mechanism of DR has not been elucidated yet. Therefore, a label-free quantitative proteomics was applied to conduct a systematic and comprehensive proteomic analysis on DR after 6 kGy γ-irradiation at different time point. Differentially abundant proteins (DAPs) with a fold-change cutoff were reported. These proteins, providing a deep understanding of the molecular response to ionizing radiation.