Genomics

Dataset Information

118

Homo sapiens


ABSTRACT: CDK9-dependent RNA polymerase II pausing controls transcription initiation

ORGANISM(S): Homo sapiens  

PROVIDER: PRJNA378674 | ENA |

REPOSITORIES: ENA

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Publications

CDK9-dependent RNA polymerase II pausing controls transcription initiation.

Gressel Saskia S   Schwalb Björn B   Decker Tim Michael TM   Qin Weihua W   Leonhardt Heinrich H   Eick Dirk D   Cramer Patrick P  

eLife 20171010


Gene transcription can be activated by decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is achieved remains unclear. Here we use a 'multi-omics' approach to demonstrate that the duration of polymerase pausing generally limits the productive frequency of transcription initiation in human cells ('pause-initiation limit'). We further engineer a human cell line to allow for specific and rapid inhibition of the P-TEFb kinase CDK9, which is implicated  ...[more]

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