Project description:Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components. In this study we apply RNA-seq analysis to healthy human monocytes exposed to oxidized Low Density Lipoprotein (oxLDL), in vitro.
Project description:Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components. In this study we apply epigenetic, H3K27ac and H3K4me3 ChIP-seq, analysis to healthy human monocytes exposed to oxidized Low Density Lipoprotein (oxLDL), in vitro.
Project description:Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components such as Beta glucan or the BCG vaccine. Here we characterize the transcriptional events following oxidized low-density lipoprotein (oxLDL) duced trained innate immunity using RNA-seq data.
Project description:scRNAseq of monocytes from in vitro Trained immunity experiments stimulated by β-glucan (BG), uric acid (UA), muramyl dipeptide (MDP), oxidized low-density lipoprotein (oxLDL), or RPMI-Control, and respective samples restimulated with Lipopolysaccharide (LPS).
