Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sequencing of RNA fragments immunoprecipitated by anti-Flag in ALKBH5 overexpression NOMO1 cells

ABSTRACT: Sequencing of RNA fragments immunoprecipitated by anti-Flag in ALKBH5 overexpression NOMO1 cells

PROVIDER: PRJNA605453 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Sequencing of RNA fragments immunoprecipitated by anti-Flag in ALKBH5 overexpression NOMO1 cells

Project description:To identify the direct mRNA targets of ALKBH5, we conducted RIP-seq with total RNA samples from ALKBH5 overexpression NOMO1 cells

2020-05-26 | GSE144963 | GEO

Sequencing of RNA fragments immunoprecipitated by anti-Flag in CASC3 or MTDH overexpression breast cancer cells

Project description:Sequencing of RNA fragments immunoprecipitated by anti-Flag in CASC3 or MTDH overexpression breast cancer cells

| PRJNA641682 | ENA

Sequencing of RNA fragments immunoprecipitated by anti-Flag in CASC3 or MTDH overexpression breast cancer cells

Project description:RNA immunoprecipitated sequencings were conducted from CASC3 or MTDH overexpression breast cancer cells

2020-06-25 | GSE153181 | GEO

Flag-ALKBH5 and FTO interactome

Project description:The interacted proteins of ALKBH5 and FTO identified by IP-mass

2022-11-03 | MSV000090641 | MassIVE

ALKBH5 is a New Mammalian RNA Demethylase

Project description:N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as a new mammalian demethylase that oxidatively removes the m6A modification in mRNA in vitro and inside cells. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1552 differentially expressed genes which cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. We show that Alkbh5-deficiency impacts the expression levels of some of these mRNAs, supporting the observed phenotype. The discovery of this new RNA demethylase strongly suggests that the reversible m6A modification plays fundamental and broad functions in mammalian cells. RNA-seq in two cell types

2013-02-15 | E-GEOD-40132 | biostudies-arrayexpress

Flag-Sae2 ChIP seq in yeast

Project description:Flag-tagged Sae2 was immunoprecipitated from G1 or S phase yeast cells, either untreated or treated with camptothecin

2018-11-21 | GSE122782 | GEO

Small RNA-seq of Ago1 and Ago1x immunoprecipitated samples

Project description:Purpose: To identify small RNAs interacting with Ago1x Methods: FLAG-HA tagged Ago proteins were immunoprecipitated and associated miRNAs were isolated using miRNeasy Mini kit (Qiagen) Results: Interaction of Ago1x with miRNAs is comparable to that Ago1

2019-05-31 | GSE130608 | GEO

ChIP-seq of PtrVCS2 fuse FLAG overexpression plants

Project description:ChIP-seq was carried out with FLAG antibody using stem cambium tissues from PtrVCS2-FLAG overexpression plants

2022-11-11 | GSE201005 | GEO

Sequencing of mRNA with N6-methyladenosine (m6A) modification in AML cells upon ALKBH5 knockdown or overexpression

Project description:To identify the potential mRNA targets of ALKBH5, we conducted m6A-seq with mRNA samples enriched from AML cells with ALKBH5 knockdown or overexpression

2020-05-26 | GSE134763 | GEO

Chromatin immunoprecipitation of human HEK293 cells with flag-tagged IMP1 antibody

Project description:Isolation of IMP1 bound mRNAs. Flag-tagged IMP1 was expressed in HEK293 cells. Flag tagged IMP1 was immunoprecipitated and mRNAs isolated. As controls HEK293 cells that do not express Flag-tagged IMP1 was included.

2006-12-31 | E-MEXP-841 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data