Project description:Here, we adapted and improved our FASTBAC-Seq method originally designed in Helicobacter pylori to investigate T1TAs in the model organism Escherichia coli. Our approach combines a life and death selection with deep-sequencing to assess the killing capability of a toxin and obtain an overview of single-nucleotide substitutions suppressing the toxin expression or activity in absence of its antitoxin. As a proof of concept, we revisited the regulation of the plasmidic hok/Sok T1TA system.

2022-10-06 | GSE207195 | GEO

Project description:Anilis and Subulata raw reads transcriptome

| PRJNA286257 | ENA

Turnera subulata x Turnera krapovickasii

Project description:Turnera subulata x Turnera krapovickasii Map

| PRJNA80621 | ENA