ArrayExpressapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-14791/E-GEOD-14791.sdrf.txtftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-14791/E-GEOD-14791.README.txtftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-14791/E-GEOD-14791.raw.1.zipftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-14791/E-GEOD-14791.idf.txtftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-14791/E-GEOD-14791.processed.1.zipftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-14791/E-GEOD-14791.eSet.rprimaryOK200application/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE14nnn/GSE14791/suppl/filelist.txtftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE14nnn/GSE14791/suppl/GSE14791_RAW.tarmirrorOK20000150TranscriptomicsAnna TomasSus Scrofatranscription profiling by arrayhttps://www.ebi.ac.uk/arrayexpress/experiments/E-GEODemail@example.comArrayExpressmediastinal lymph nodewhole bloodGrowth Protocol - Mediastinal lymph nodes were collected at necropsy, immediately frozen in liquid nitrogen and kept at -80ºC until usage
Sample Processing - 24 seven-day-old, CDCD piglets were used; a first group of pigs (n=8) was kept as un-inoculated controls and the rest of pigs (n=16) were oronasally inoculated with 105.2 TCID50 of the Burgos isolate of PCV2.
Hybridization - Following fragmentation, 10 ug of cRNA were hybridized on GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Labeling - Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (One-Cycle Target Labeling kit, Expression Analysis Technical Manual, 2001, Affymetrix).
Nucleic Acid Extraction - Total RNA was extracted with the RiboPure kit (Ambion Cat #1924) according to the manufacturer's instructions.Assay Data Transformation - ID_REF = <br>VALUE = RMA signal intensity
Feature Extraction - The data were analyzed with the Data Mining Tool (DMT) from Affymetrix. RMA was used as normalization method.
Image Adquisition - Chips were scanned using the Genechip Scanner 3000150AA407358, Warthogs, Cas1, Carnitine acetylase, Node, cycline, determination, DmcyclinE, fond, Wart Hog, SCF receptor activity, Cs-1, cycE, Xkl-1, CycEI, l(2)br37, CG11121, CYCLE, Hog, cdi7, CrAT, method, KL receptor activity, old, Gsfsco1, Ccne, cyclinE, manganese catalase activity, scfr, domestic cat, DmelCG11121, method used in an experiment, Cdi7, CDI7, SCO5, NRSF, SCO1, wild boar, caperase activity, Gsfsow3, SCFR, Gsfsco5, 2210418N07, SOW3, Lymph, Fdc, Suidae, somda, CYCE, Genomes, Rests, CT21282, DmelCG3938, N, CyclE, 3938, CatA, CATA, labeling, W, DmcycE, SO, l(2)k05007, DmelCG6871, dm-cycE, haem catalase activity, Catl, grupos, sample, boars, swine, Hogs, Bs, CG3973, cats, liquid, Sl, So, Drl, AI315345, catalase-peroxidase activity, proto-oncogene c-Kit, equilase activity, Wart, DMCATHPO, Phacochoerus, grupo, total RNA extract, heme catalase activity, pig, pigs, 2610008J04Rik, TelN, Tr-kit, BC058979, bacterial catalase-peroxidase activity., Sus scrofus, 220.127.116.11, br37, Cyc E, Carnitine acetyltransferase, AI195249, Mdu, group, A330009E03Rik, PBT, l(2)k02514, catalase reaction, DmCycE, BG:DS07108.3, Stickstoff, XBR, mda, KIT ligand receptor activity, Felis domesticus, chemical analysis, kl1-A, Wart Hogs, CyeE, KIT, CAT, l(2)05206, GAT, ami, XKrk1, ACGNAT, CS1, optidase activity, tyrosine-protein kinase Kit, pbt, DmelCG3973, c-KIT, Cas-1, l(2)k02602, Lymph Node, med, l(2)35Dd, hydrogen-peroxide:hydrogen-peroxide oxidoreductase activity, Rest, CG6871, azote, whole genome, kit, cat, CD117, study protocol, Felis silvestris catus, sample population, Gm393, nitrogeno, Pigs, plan specification, D-CycE, c-kit, nitrogen, l35Dd, C-Kit, Cat01, Gm689, Ssm, Nodes, xkl-1, ResT, microarray, assay, 7N, DROCATHPO, CG3938, groupe, U00145, bs36h11.y1, Gruppe, krk1measuring, data, Procedures, DmelCG10360, Ref(2)P, ALY, Mining, ref, ref(2)p, Procedure, feature_extraction, any method, Ref2P, ref2p, Techniques, method, ref(2)Po2, CG8374, Method, Ref(2)p, p62, THOC4, method used in an experiment, Studies, Technique, IMAGE., s048103, ALY|REF, 0481/03, anon-WO0118547.317, EPB49, ALY/REF, Methodological, study assay, Methodological Study, plan specification, Study, BEF, data analysis, data processing, Methodological Studies, Data, Text, DMT, Dmt, scientific observation, Text Mining, REF, CG10360, DmelCG8374, ref(2)Pnportion of blood, data, Node, whole blood, Lymph Node, vertebrate blood., Nodes, Lymphportion of blood, Porcine circovirus Type II., data, Node, whole blood, Lymph Node, Porcine circovirus Type II, vertebrate blood, structure, content, Nodes, Porcine circovirus type 2, composition, Porcine circovirus-2, compositionality, composed of, Lymph0.00.00.00.00.0046267735965453425trueExpression data from mediastinal lymph nodes and blood samples of piglets experimentally infected with PCV2This SuperSeries is composed of the following subset Series: GSE14758: Expression data from mediastinal lymph nodes of piglets experimentally infected with porcine circovirus type 2 (PCV2) GSE14790: Expression data from blood samples of piglets experimentally infected with porcine circovirus type 2 (PCV2) Refer to individual Series2009-12-302011-06-10E-GEOD-147919823