ArrayExpressapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-195/E-MEXP-195.sdrf.txtftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-195/E-MEXP-195.README.txtftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-195/E-MEXP-195.raw.1.zipftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-195/E-MEXP-195.idf.txtprimaryOK20000122TranscriptomicsLemonnier Gaëtan(Make:Axon Instruments,Model:GenePix 4000A)Default scanner softwareArabidopsis Thalianatranscription profiling by arrayhttps://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-195glemonni@evry.inra.frArrayExpressGrowth Protocol - Arabidopsis Col0 Seedlings were cultured on a Murashige and Skoog medium as described by Estelle and Somerville (1987) but without sucrose. Seeds were arrayed individually on a Nylon filter (BioTechnoFix) on 9cm petridishes and incubated for 72h at 4°C, followed by 4h at 20°C, under white light (100µmol/m2s). Plates were wrapped in 3 layers of aluminum foil and incubated at 20°C for the indicated time periods. For the isoxaben treatment, the Nylon filters with the seedlings were transferred under green safe light to a medium supplied with 4nM isoxaben in a final concentration of 5,6mM DMSO or to a control medium containing the same concentration of DMSO. Upon harvest, hypocotyls were dissected out under green light (approximately 30 hypocotyls per experiment), the dissection took approximately 10 min per time point. 16 harvests were pooled, in total 475 hypocotyls, for each time point. RNA was extracted using the trizol reagent (Invitrogen). Sample Processing - For the isoxaben treatment, the Nylon filters with the seedlings were transferred under green safe light to a medium supplied with 4nM isoxaben in a final concentration of 5,6mM DMSO or to a control medium containing the same concentration of DMSO Hybridization - Slide preparation In a Coplin jar, prepare 50 ml of pre-hybridization solution : SSC 20 X 12,5 ml BSA 10 % 5 ml SDS 10 % 0,5 ml H2O 32 ml Pre-heat this solution at 42 °C and préhybridize slides at 42 °C for at least 45 mn. Wash the slides in milli-Q water, and then isopropanol. Blow-dry the slides. THE SLIDES MUST BE USED WITHIN 1 HOUR! Hybridization Prepare 2X hybridation mix (50 µl per slide) : SSC 20x 25 µl SDS 10% 1µl Formamide 25 µl Denature the probe 1 mn at 95°C and keep on ice. Add one volume of 2X hybridation mix, mix and centrifuge. Put the slide in the Corning hybridization chamber, and place a cover slip on it. Pipet the probe between the slide and the cover slip. Put 10 µl of water in the two holes and close the chamber. Incubate in a water bath at 42 °C over-night (15h) Washes Soak in 2X SSC, 0,2 % SDS at 42°C to remove the cover slip, then wash in : - 1X SSC, 0,2 % SDS (42°C) 4 min - 0,2X SSC, 0,2 % SDS 4 min - 0,05X SSC 4 min Blow dry the slide or centrifuge for 2 min at 600 rpm. Store in a dry and dark place until scanning. Labeling - 5 µg of aRNA (8µl) mixed with 2 µl of random nonamers 1µg/µl and 0.5 µl of RNAse Out 40 U/µl, denatured 10 min at 70 °C and chilled on ice. The followig components were added to the sample: 4 µl of first strand buffer 5 X, 1 µl of 10 mM dNTP/2mM dCTP, 2 µl of 0.1 M DTT, 1.5 µl of Cy3-dCTP (Amersham Pharmacia Biotech, 25nmol tube, PA5502), 1 µl of SuperScript II RT 200U/µl. Then it was incubated at 42°C for 2.5 hours and chilled on ice. The sample was denaturated by adding 2 µl of NaOH 2.5M and incubated at 37 °C for exactly 15 min, then was added 10 µl of 2M MOPS and put on ice. The dyes were purified with the QIAquick PCR Purification Kit (QIAGEN). Nucleic Acid Extraction - Plant tissue was ground to fine powder using liquid nitrogen in a 2mL eppendorf tube. The samples were homogenized in TRIzol Reagent (Invitrogen) : 1 mL for 50-100 mg of plant tissue, by vortexing and incubated at room temperature for 5 min. Chloroform (200µl/1mL TRIzol) was added and the capped tubes were hand-shaken for 15 sec. They were incubated for 2-3 min, then centrifuged for 15 min at 11000 rpm at 4°C. The aqueous phase was transferred to a new 1.5 mL tube and 500ul of isopropanol was added. The samples were incubated for 10 min at room temperature then centrifuged for 10 min at 11000 rpm at 4°C. The supernatant was removed and the pellet was washed with 1 mL of 75% ethanol in DEPC-treated water. The samples were centrifuged for 5 min at 11000 rpm at 4°C. The pellet was air-dried for 10 min at room-temperature, then resuspended in 500 µl DEPC-treated water, and precipated with 2 volumes of 100 % ethanol and 1/10 V of Sodium acetate 3M, pH5,2. The samples were precipitated 30 min at -20 °C and centrifuged for 15 min at 10000 rpm at 4°C. The supernatant was removed, the pellet was air-dried and resuspended in DEPC-treated water. The RNA solution was purified on Qiagen RN-Easy column. The RNA quality was then acessed on Agilent Bioanalyser following the manufacturer's protocol and the quantity is determined by Ribogreen.Assay Data Transformation - Analysis of 2 technical repeats The statistical analysis was based on two dye-swaps, i.e. four arrays each containing the 24576 GSTs and 384 controls. The controls were used for assessing the quality of the hybridizations but were not included in the statistical tests or the graphical representation of the results shown in the Results. For each array, the raw data comprised the logarithm of median feature pixel intensity at wavelength 635 nm (red) and 532 nm (green). No background was subtracted. In the following description, log-ratio refers to the differential expression between leaves and flowers. It is either log2(red/green) or log2(green/red) according to the experiment design. An array-by-array normalization was performed to remove systematic biases. First, we excluded spots that were considered badly formed features by the experimenter. Then we performed a global intensity-dependent normalization using the loess procedure (see (Yang et al., 2002) to correct the dye bias. Finally, on each block, the log-ratio median is subtracted from each value of the log-ratio of the block to correct a print-tip effect on each metablock. To determine differentially expressed genes, we performed a paired t-test on the log-ratios. The number of observations per spot varies between 2 and 4 and is inadequate for calculating a gene-specific variance. For this reason we assume that the variance of the log-ratios is the same for all genes and we excluded 256 spots displaying extremes of variance (too small or too large). The raw P-values were adjusted by the Bonferroni method, which controls the Family Wise Error Rate (FWER)(Ge et al., 2003). Image Adquisition - Scanning All the slides for a same project are scanned at a constant PMT power (i.e. 650V)126-Dimethoxy-N-(3-(1-ethyl-1-methylpropyl)-5-isoxazolyl)benzamide, EL 107, cellulose formation, polarized cell growth, alpha-Cellulose, Avicel, Rayophane, developmental stage, diaspore (broad), wall of organ, GRO:0005339, INSDC_feature:misc_RNA, Cell, cellulose biosynthesis, N-(3-(1-Ethyl-1-methylpropyl)-5-isoxazolyl)-2, cell growth in one dimension, Alphacel, 6-dimethoxybenzamide, 種子 (Japanese, cellulose synthesis, Experiment, exact), Polyanhydroglucuronic Acid, 6-dimethoxy-N-[3-(3-methylpentan-3-yl)-1, Embryos, responsivity, C18H24N2O4, organ wall, feed, Hypocotyls, 2, slow, cell morphogenesis by unidimensional growth, Seed, El-107, cell growth in one dimension., N-(3-(1-ethyl-1-methylpropyl)-5-isoxazolyl)-2, Walls, slow speed, Cell Walls, polymer, 壁, study, Embryo, Acid, Polyanhydroglucuronic, reactivity, Wall, seed, Heweten, distinct, growth pattern, 器官墙(wall of organ), 2-oxazol-5-yl]benzamide, non-developmental growth, cell elongation, alpha Cellulose, Plant, cell growth along one axis, Plant Embryo, Plant Zygote, Zygote, dark, Plant Embryos, polar cell growth, Sulfite Cellulose, 器官墙(organ wall), Polymer, semilla (Spanish, Plant Zygotes, Accelerations, Zygotes, stage, response, cellulose anabolism, wall, pyrene (narrow)6-Dimethoxy-N-(3-(1-ethyl-1-methylpropyl)-5-isoxazolyl)benzamide, EL 107, C18H24N2O4., Cresses, Effects, Longterm, 2-oxazol-5-yl]benzamide, A., Arabidopsis thaliana, Mouse-ear Cress, Longterm Effect, thaliana, A. thaliana, Columbia-0, Long-Term, Mouse-ear Cresses, Mouse-ear, Long Term, Longterm Effects, N-(3-(1-Ethyl-1-methylpropyl)-5-isoxazolyl)-2, Cardaminopsis, 6-dimethoxybenzamide, transcription profiling, period, Arabidopsis, Arabidopsis thalianas, 6-dimethoxy-N-[3-(3-methylpentan-3-yl)-1, A. thalianas, Long Term Effects, thalianas, Hypocotyls, 2, Cress, Long-Term Effect, Mouse ear, El-107, Arabidopses, Effect, Long-Term Effects, time, N-(3-(1-ethyl-1-methylpropyl)-5-isoxazolyl)-2, gene expression profilingMethane, CPD photolyase activity, Ribonucleic, Thiofaco M-50, DIMETHYL SULFOXIDE, IL1BC, PhrB photolyase activity, Ethanol Absolute, A., CASP-1, nutrient medium, BOUND WATER, Visible Light, oxidane, Long Term, trichloro-, Silent Spirit, Cardaminopsis, HOH, SDS, KL receptor activity, 1-(5-O-phosphono-D-ribofuranosyl)-1H-imidazol-5-amine, Anhydrous Sodium Acetate, Abc8, Method, Hot, A. thalianas, 80%, SCO5, SEC, 2, SCO1, El-107, outer pigmented layer of retina, Il1bc, Gsfsow3, epithelium, Non Polyadenylated, treatment, ADHD, Grain, InChIKey=LFQSCWFLJHTTHZ-UHFFFAOYAB, N, HAND, sec, Tissue, dimethyl sulphoxide, Plant Embryo, ORF19, aluminium atom, Aminoethanol, W, Denatured Ethanol, Mouse-ear, IL-1BC, foot, hand, Dehydrated ethanol, pigment epithelium of retina, 组织部分(tissue portion), dehydrated, h, Beta-Ethanolamine, column, method used in an experiment., Ethyl Alcohol & Water, dxnph, AA409961, anatomical tube, 70%, sample, m, Sprouted, pigmented retina epithelium, Bs, Dimethylsulfoxid, spiritus vini, 6-Dimethoxy-N-(3-(1-ethyl-1-methylpropyl)-5-isoxazolyl)benzamide, Beta-Hydroxyethylamine, DMSO, Radiation, millidalton, Caspase-1 subunit p10, 95%, dmso, Beta-Aminoethanol, 10^[-3], Sodium hydroxide, Longterm Effect, Light, terminal segment of free upper limb, Ethanol Absolute Bp, trait, Procedure, 2H2, add, Absolute Alcohol, PBT, (CH3)2SO, H2O, Plant Sprouts, LIGHT, paw, 96%, Stickstoff, dipyrimidine photolyase (photosensitive), Ribonucleic acids, simple tissue, 1110049F14Rik, [OEtH], Hydrogen Oxide, Isopropanol, PCR, Acid, Seeds, aluminio, HVEML, medium, GLI3FL, alpha-D-Glucopyranoside, InChI=1/Al, pooled, Plant Zygote, reagent, InChI=1/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11, Shwachman syndrome, Methodological, sodium hydrate, Ethyl Hydroxide, study protocol, Pdn, Ethylol, ANX2LG, Methylcarbinol, alcohol etilico, 1H3, 管道, polymerase chain reaction, 1-3H2/t4-, Aminoimidazole ribotide, Denatured Alcohol Sd-1, Seedling, Grains, nitrogen, GP11, 50%, hand region, duct, Denatured Alcohol Cd-10, fore paw, White, forefoot, InChIKey=CZMRCDWAGMRECN-UGDNZRGBBE, Plant Sprout, forelimb autopodium, Effects, Denatured Alcohol, etanol, beta-D-Fruf-(2<->1)-alpha-D-Glcp, dimethylsulfoxyde, Sodium Acetate Trihydrate, C2H6OS, Ethanol 200 Proof, 13-20H, deoxyribodipyrimidine photolyase activity, stratum pigmentosum retinae, agua, period, Caucasian, 40%, AIR, Sprouted Seed, thalianas, Gene Products, Tecsol C, U/l, AI326420, whitish, plantae, DNA-PKcs, sulfinylbis(methane), FAM39E, Cresses, Longterm, Tecsol, Isopropyl, HYRC1, PCE-2, 4733401P19Rik, Cal1l, Zygote, Sodium Acetate, Long-Term, Alcool Etilico, Study, dimetil sulfoxido, C2H5OH, Anhydrol, C12H22O11, AU019811, 30%, Caspase-1 subunit p20, Cress, ETA, Ca[1], TR2, slip, 426, Anhydrous, Absolute Ethanol, CAL12, proto-oncogene c-Kit, RNA, 42C, 1-(5'-Phosphoribosyl)-5-aminoimidazole, Arabidopsis thaliana, Denatured Alcohol Sd-3a, InChI=1/H2O/h1H2, RNS, CE-2, Sprout, CD258, buffer, Glycinol, Wash2, Wash1, Experiment, CAL1L, Denatured Alcohol Cd-5a, 20%, sacarosa, Temperatures, dry, KIT ligand receptor activity, Acetate, Long Term Effects, alcool ethylique, MIXL, Sodium, C2H6O, soda lye, Beta-Aminoethyl Alcohol, Bph, Ethyl Hydrate, Sprouts, RPE, Denatured Alcohol Sd-28, mDa, Denatured, Caswell No, thaliana, dimethyl sulfoxide, SWDS, Shwachman-Bodian-Diamond syndrome, IL-1 beta-converting enzyme, Ribonucleic Acid, azote, Interleukin-1 beta-converting enzyme, CD117, retinal pigment epithelium, Longterm Effects, p. pigmentosa retinae, Hands, plan specification, Rubbing, 10%, Photoradiations, Aluminum-27, C-Kit, Denatured Alcohol Sd-17, Pancreatic insufficiency and bone marrow dysfunction, 10+, Ssm, forepaw, Aetznatron, Ethylolamine, deoxyribonucleate pyrimidine dimer lyase (photosensitive), Attention Deficit Hyperactivity Disorder, [OH2], 13Al, JAR, Pyro, Grain Alcohol, AU023367, 11-, Pflanze, Diluted, pigmented epithelium, 1-hydroxyethane, N-(3-(1-Ethyl-1-methylpropyl)-5-isoxazolyl)-2, InChIKey=XLYOFNOQVPJJNP-UHFFFAOYAF, viridiplantae, 6-dimethoxybenzamide, OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O, Ethanol, Arabidopsis thalianas, Gsfsco1, forelimb autopod, Alkohol, Heat, Aluminium-27, Effect, Gsfsco5, RNA Gene Products, MIX, SOW3, portion of tissue, Malted Grain, reference sample, aluminum, Ethyl, Mouse-ear Cress, pigmented retina, min, Jaysol S, Aethanol, [CH2Me(OH)], DNA cyclobutane dipyrimidine photolyase activity, Thanol, XRCC7, Methodological Studies, ICE, disease management, dihydridooxygen, P11, 简单组织, Dehydrated, dihydrogen oxide, Natriumhydroxid, hydroxyde de sodium, Arabidopses, Sl, time point, Long-Term Effects, forefoot of quadruped, Dissections, PRE, dimethyli sulfoxidum, Alcohol, iCE, AW413978, ribose nucleic acid, 12+/m1/s1, p10, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, methylsulfinylmethane, 5%, ribonucleic acids, aqua, p11, AI836084, white, Tr-kit, A. thaliana, 5-, pigmented retinal epithelium, Isopropyl Alcohol, beta-D-fructofuranosyl, retinal pigment, Ribonukleinsaeure, Rubbing Alcohol, tissue portion, Hypocotyls, pentosenucleic acids, CLP11, InChIKey=XAGFODPZIPBFFR-UHFFFAOYAX, Seed, 6-, WASH, KIT, retinal pigment layer, Aluminum 27, Visible Radiations, 组织部分(portion of tissue), tyrosine-protein kinase Kit, MILD1, (methanesulfinyl)methane, Visible Radiation, table sugar, NaOH, HSDB 531, unit per litre, EtOH, 1-alpha-D-Glucopyranosyl-2-beta-D-fructofuranoside, Alcare Hand Degermer, retinal pigmented epithelium, AI854843, P45, DNAPK, Plant, hydrogen hydroxide, mmu, 7-, Envision Conditioner Pdd 9020, Methodological Study, ANX2L, acqua, Trichloromethane, phr A photolyase activity, Arabidopsis, DNA-photoreactivating enzyme, p45, Hot Temperatures, DOXNPH, 8+, Ethanol Extra Pure, Wasser, 7N, time, Denatured Alcohol Cd-5, Etanolo, EL 107, Procedures, 酪氨酸蛋白激酶试剂盒, SCF receptor activity, USAF EK-1597, 9-, photoreactivating enzyme activity, Xt, [Al], DNAPDcs, Reagent Alcohol, Aethylalkohol, method, AL024248, 6-dimethoxy-N-[3-(3-methylpentan-3-yl)-1, sodium hydroxide, DNPK1, Ethyl Alcohol Anhydrous, Studies, Sacharose, 原癌基因c-Kit, stratum pigmentosa retinae, Malted, light, SCFR, N-(3-(1-ethyl-1-methylpropyl)-5-isoxazolyl)-2, C8H14N3O7P, 解剖管, S(O)Me2, Colamine, Fdc, [H]O[H], deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, Saccharose, Absolute, p460, Trihydrate, Selb, 5'-Phosphoribosyl-5-aminoimidazole, labeling, soude caustique, Visible, eau, dark, scid, Non-Polyadenylated RNA, Ethanol Anhydrous, Denatured Alcohol Sd-39b, Denatured Alcohol Sd-39c, RANDOM, 5-Amino-1-(5-phospho-D-ribosyl)imidazole, Synasol, Zygotes, Long-Term Effect, liquid, Mouse ear, 2 Propanol, Controlled, Controlling, Acetate Trihydrate, Malted Grains, milli unified atomic mass unit, Jaysol, Alcohol Anhydrous, stratum pigmentosum (retina), GLI3-190, Denatured Alcohol Sd-13a, yeast nucleic acid, fore-paw, Embryos, C18H24N2O4, deoxyribonucleic photolyase activity, caustic soda, Algrain, hybridization_chamber, HNaO, CES2A1, Dimethyl sulfoxide, 1-(5'-phosphoribosyl)-5-aminoimidazole, forefeet, Random selection by shearing, Radiations, Ethyl Alcohol, Embryo, ribonucleic acid, Temperature, c-KIT, 1-(5-Phospho-D-ribosyl)-5-aminoimidazole, waterbath, 2-oxazol-5-yl]benzamide, photolyase activity, growth medium, Non Polyadenylated RNA, Photoradiation, Non-Polyadenylated, InChI=1/C2H6O/c1-2-3/h3H, CGI-97, Mouse-ear Cresses, beta-D-fructofuranosyl alpha-D-glucopyranoside, Plant Embryos, sample population, LTg, nitrogeno, Interleukin-1 beta convertase, hydroxyethane, Cane sugar, Denatured Alcohol Sd-23a, Plant Zygotes, concentration, fore foot, Spirit, Aluminium 27, CCO, Sprouted Seeds, 3.4.22.36, dimethyl sulfur oxidePetals, Extended Families, Networks, Scientific Bias, Bru, Plant Sepal, PLIP, Materials, Extended, Kinship, Raw, determination, ectodin, vif, Extended Family, A Genes, Type 2A-interacting protein, inadequate, Sepal, Plant Bloom, Method, Epidemiologic Biase, Plant Styles, Life Cycle, Ecological, Blooms, Plant Carpal, Bloom, HTATIP, Family Life Cycle, Svc, A, Ecological Biases, Kinship Network, GSTS, Plant Ovary, bA325O24.3, sor Genes, Q, hypoplasia, USAG-1, C6orf207, number of, Plant Anther, Pistil, Corolla, SURGICAL AND MEDICAL PROCEDURES, Corollas, cPLA2, Methodological Studies, Statistical Biases, scientific observation, RED, dye, Ecological Bias, Systematic, Family Life Cycles, Epidemiologic Biases, TIP, ratio, 0610006G05Rik, Scientific, ZC2HC5, Epidemiologic, Plant Anthers, Calyx, GASP, extra or missing physical or functional parts, Ecological Fallacies, HTATIP1, trait, Procedure, Pistils, results, CSA2, S-adenosyl-L-methionine:phenol O-methyltransferase activity., Outcome Measurement Errors, any method, Putative MAPK-activating protein PM10, Experimental, batch, count, Plant Corollas, Ovaries, Carpals, Genetic Materials, bA325O24.4, median, Plant Blooms, Truncation Bias, TIP60, Filiation, Genetic Material, Anther, vif Genes, Q Genes, Bias, Reconstituted Family, Plant Corolla, proportionality, TSEPA, Plant, expanded, rate, Filaments, Plant Calyx, Methodological, Outcome Measurement, Methodological Study, Experimental Bias, Life Cycles, data analysis, Outcome Measurement Error, Reconstituted, Plant Stigma, enlarged, Material, Sostl, Wise, microarray, Blossoms, Cistron, Anthers, TIP41, Blossom, big, Stigma, C6orf190, Family Member, Procedures, Ecological Fallacy, vif Gene, Truncation, number, IMAGE, Systematic Bias, Gene, sor Gene, Network, Calyxs, Plant Stigmas, presence, ESA1, Intervention or Procedure, Filament, SPOT, large, method, reduced, method used in an experiment, Stigmas, Studies, Del(8)44H, tiny, spot, Styles, Fallacies, hYAK3-2, proportion, Genetic, interventionDescription, Research, Aggregation, uniform, Flower Filaments, Interventional, Style, study assay, Study, data processing, great, has or lacks parts of type, Plant Sepals, Plant Stamen, Kinship Networks, Family, A Gene, statistical analysis, Stamens, Biase, DYRK5, small, measuring, Intervention Strategies, Fallacy, constant, data, Carpal, Family Research, Ovary, Plant Carpals, Truncation Biases, Reconstituted Families, sor, Cistrons, mereological quality, Family Members, count in organism, Plant Style, Experiment, Stepfamily, chemical analysis, Errors, background, Q Gene, Plant Ovaries, Flower Filament, Intervention, Genes, Col4a-1, underdeveloped, Sepals, Biases, Plant Calyxs, REDK, introduction, plan specification, Flower, Stamen, Error, Aggregation Bias, Plant Stamens, Families, Plant Petals, Statistical Bias, cardinality, quotient, 数据, Plant Petal, assay, quantitative, PMT, Statistical, Relatives, Stepfamilies, Petal, presence or absence in organism40.00.00.0037014188772362749.78162521702981E-502dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0genome_assembly~0sc-experiments~0taxonomy~1ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~1omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0lineage-covid19~0gpmdb~0metagenomics~0pfam~0varsite~0true6.352634755264746E-5trueTranscription profiling of Arabidopsis Col-0 hypocotyls time series treated with isoxaben vs untreated samples1. Study the molecular basis of the growth acceleration observed in hypocotyl cells. We previously have observed that cell elongation takes place in two distinct phases (Refregier et al., 2004). A slow growth phase during which a thick polylamellated wall is deposited and a rapid growth phase during which cell wall polymers are extensively remodelled. In dark-grown hypocotyls the slow growth phase takes place during the first 48h after seed-imbibition synchronously in all cells. At 48h after imbibition, cells at the basis of the hypocotyl undergo a growth acceleration, this acceleration follows an acropetal gradient along the hypocotyl. In this experiment, we investigated the changes in transcript abundance that accompany this sudden increase in growth rate. 2. Study the feed-back mechanisms involved in the coordination between cellulose synthesis and the cell elongation. The inhibition of cellulose using chemical inhibitors also inhibits cell elongation. In the same study (Refregier et al., 2004), we have observed that the effect of the cellulose synthesis inhibitor isoxaben on cell elongation is different dependent on the growth stage. When applied during the slow growth phase, cells continue to elongate slowly and do not show the growth acceleration at 48h after imbibition. Surprisingly, when applied after the growth acceleration, isoxaben does not inhibit subsequent growth. In this study we compared the effects of isoxaben on the transcript profiles before and after the growth acceleration. This should inform us about the response of the hypocotyl cells to the inhibition of cellulose and should provide insights into the molecular events that underly the observed coupling between cellulose synthesis and cell elongation.2005-01-012014-05-02E-MEXP-1953702