{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":[null],"species":["Homo sapiens (human)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD1038"],"repository":["bioimages"],"figure_sub":["Protocols","Study Component","organisation","Study Protocols"],"pubmed_authors":["Christian Gabriel"],"additional_accession":[]},"is_claimable":false,"name":"Circadian Imaging of PER and CRY Proteins in U-2 OS cells","description":"U-2 OS knock-in cells were genereated using CRISPR to express CRY1, CRY2, PER1 PER2 fused to either mScarlet-I or mClover3 from the endogenous promoter. All cells were transduced with H2B-iRFP using lentivirus to allow segmentation and tracking of nuclei. \n\nAdditionally, cells were transduced with lentivirus expressing shRNA against either FBXL3 (pGIPZ V2LHS_254986) or a nontargeted control shRNA. \n\n===Raw Data===\n\n==Dataset Dex==\nCells of selected clones were imaged for ~3days after synchronization with dexamethasone (1µM, 20min) at an imaging interval of 1 picture per hour\n==Dataset CHX/Main==\nIn 3 experiments, cells were imaged for 68 hours with an imaging interval of 1 picture per hour.\nAfter 44-48 hours, 20 µg/ml CHX was added to a subset of cells to inhibit protein production.\nFor as","dates":{"release":"2024-02-09T00:00:00Z","modification":"2024-02-09T08:55:41.507Z","creation":"2024-02-09T08:55:41.507Z"},"accession":"S-BIAD1038","cross_references":{}}