bioimagesUnknown12Homo sapiens (human)https://www.ebi.ac.uk/biostudies/studies/S-BIAD1038bioimagesProtocolsStudy ComponentorganisationStudy ProtocolsChristian GabrielfalseCircadian Imaging of PER and CRY Proteins in U-2 OS cellsU-2 OS knock-in cells were genereated using CRISPR to express CRY1, CRY2, PER1 PER2 fused to either mScarlet-I or mClover3 from the endogenous promoter. All cells were transduced with H2B-iRFP using lentivirus to allow segmentation and tracking of nuclei. Additionally, cells were transduced with lentivirus expressing shRNA against either FBXL3 (pGIPZ V2LHS_254986) or a nontargeted control shRNA. ===Raw Data=== ==Dataset Dex== Cells of selected clones were imaged for ~3days after synchronization with dexamethasone (1µM, 20min) at an imaging interval of 1 picture per hour ==Dataset CHX/Main== In 3 experiments, cells were imaged for 68 hours with an imaging interval of 1 picture per hour. After 44-48 hours, 20 µg/ml CHX was added to a subset of cells to inhibit protein production. For as2024-02-09T00:00:00Z2024-02-09T08:55:41.507Z2024-02-09T08:55:41.507ZS-BIAD1038