bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1083bioimagesSpecimenFundingStudy ComponentorganisationBiosampleAssociationsImage acquisitionRosmarie SütterlinA. Leoni SwartPablo ManfrediSandro JakoniaBenoit-Joseph LaventieXiao YuLuisa LauerEvdoxia KaragkioziRusudan OkujavaUrs JenalTina JunnefalsePseudomonas aeruginosa breaches respiratory epithelia through goblet cell invasion in a microtissue modelWhile commensal bacteria generally respect natural barriers of the human body, pathogens are able to breach epithelia, invade deeper tissue layers and cause life-threatening infections. Pseudomonas aeruginosa, an opportunistic human pathogen, is a leading cause of severe hospital-acquired pneumonia, with mortality rates up to 50% in mechanically ventilated patients1–3. Effective colonization and breaching of lung mucosa are hallmarks of P. aeruginosa pathogenesis4. Although virulence factors and behavioral strategies of P. aeruginosa have been described5,6, it has remained unclear how this pathogen disseminates on functional mucosal surfaces, how it avoids mucociliary clearance and how it invades the tissue barrier. Using fully differentiated human lung epithelia, we demonstrate that P. ae2024-04-11T00:00:00Z2024-05-19T18:59:41.487Z2024-03-14T09:15:54.367ZS-BIAD1083